Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1128/aem.01262-24 |
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| Title (Primary) | Nitrous oxide is the main product during nitrate reduction by a novel lithoautotrophic iron(II)-oxidizing culture from an organic-rich paddy soil |
| Author | Grimm, H.; Lorenz, J.; Straub, D.; Joshi, P.; Shuster, J.; Zarfl, C.; Muehe, E.M.; Kappler, A. |
| Source Titel | Applied and Environmental Microbiology |
| Year | 2025 |
| Department | AME |
| Volume | 91 |
| Issue | 1 |
| Page From | e01262-24 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Data and Software links | https://doi.org/10.5281/zenodo.13683983 |
| Keywords | culture HP; airborne microorganisms; greenhouse gas; arsenic; chemodenitrification; nitrogen, soil |
| Abstract | Microbial nitrate reduction coupled to iron(II)
oxidation (NRFeOx) occurs in paddy soils due to high levels of dissolved
iron(II) and regular application of nitrogen fertilizer. However, to
date, there is no lithoautotrophic NRFeOx isolate or enrichment culture
available from this soil environment. Thus, resulting impacts on
greenhouse gas emissions during nitrate reduction (i.e., nitrous oxide
[N2O]) and on toxic metalloid (i.e., arsenic) mobility can
hardly be investigated. We enriched a lithoautotrophic NRFeOx culture,
culture HP (Huilongpu paddy, named after its origin), from a paddy soil
(Huilongpu Town, China), which was dominated by Gallionella (71%). The culture reduced 0.45 to 0.63 mM nitrate and oxidized 1.76 to 2.31 mM iron(II) within 4 days leading to N2O as the main N-product (62%–88% N2O-N of total reduced NO3−-N).
Nitrite was present as an intermediate at a maximum of 0.16 ± 0.1 mM.
Cells were associated with, but mostly not encrusted by, poorly
crystalline iron(III) minerals (ferrihydrite). Culture HP performed best
below an iron(II) threshold of 2.5–3.5 mM and in a pH range of
6.50–7.05. In the presence of 100 µM arsenite, only 0%–18% of iron(II)
was oxidized. Due to low iron(II) oxidation, arsenite was not
immobilized. However, the proportion of N2O-N of total reduced NO3−-N
decreased from 77% to 30%. Our results indicate that lithoautotrophic
NRFeOx occurs even in organic-rich paddy soils, resulting in
denitrification and subsequent N2O emissions. The obtained
novel enrichment culture allows us to study the impact of
lithoautotrophic NRFeOx on arsenic mobility and N2O emissions in paddy soils. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=31774 |
| Grimm, H., Lorenz, J., Straub, D., Joshi, P., Shuster, J., Zarfl, C., Muehe, E.M., Kappler, A. (2025): Nitrous oxide is the main product during nitrate reduction by a novel lithoautotrophic iron(II)-oxidizing culture from an organic-rich paddy soil Appl. Environ. Microb. 91 (1), e01262-24 10.1128/aem.01262-24 |
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