Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.dib.2025.112157 |
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| Title (Primary) | Experimental dataset on the characterization of a new photo-calorespirometry setup |
| Author | Linke, T.; Paufler, S.; Dusny, C.; Maskow, T.
; Schmid, A. |
| Source Titel | Data in Brief |
| Year | 2025 |
| Department | SOMA; MIBITECH |
| Volume | 63 |
| Page From | art. 112157 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Data and Software links | https://doi.org/10.17632/56s83cx3mp.1 |
| Keywords | Photo-Calorimetry; light adjustment/quantification; cyanobacterial phenotypes; dead cell preparation; pigment analysis; morphological characterization; thermal profiling |
| Abstract | This dataset complements the main article “Direct Real-Time Determination of Photosynthetic Efficiency by Photo-Calorespirometry Using Synechocystis sp. PCC 6803 as Model Cyanobacterium” by providing detailed experimental data, calibration procedures, and validation measurements for the photo-calorespirometry (Photo-CR) method. The data ensure precise quantification of light energy input (PI), thermal signals, and photosynthetic performance, and validate control systems essential for accurate heat-based assessment of photosynthesis in phototrophic microorganisms. Light delivery was optimized and in a dual-ampoule calorimetric setup trough calibrated LED–light guide assemblies. including distance-dependent attenuation profiles, peak (PI) determinations (20 mW), and corrections for thermal asymmetry. A photosynthetically inactive reference system was established by formaldehyde fixation of Synechocystis cells, preserving morphology and pigment content. Physiological and optical validations included Coulter counter cell-size analysis, pigment quantification, and absorption spectroscopy to confirm spectral similarity between fixed and living cells. Control datasets include thermal profiles of living versus dead cells, medium-only baselines, and light-independent heat generation. Additional measurements capture temporal variations in photosynthetic performance during multi-step light ramp experiments, normalized to biomass via Monod-based growth modeling. Furthermore, a correlation dataset from 18 independent samples links cell number, optical density (OD₇₅₀), and cell dry weight, enabling conversion between these measures. Collectively, these datasets provide a comprehensive reference for calibrating and validating Photo-CR experiments, supporting reproducible measurement of photosynthetic energy conversion in cyanobacteria. They are intended for reuse in methodological replication, adaptation to other phototrophic microorganisms, optimization of light-dependent calorimetric studies, and the development of standardized reference controls for heat-based photosynthetic efficiency assessment. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=31420 |
| Linke, T., Paufler, S., Dusny, C., Maskow, T., Schmid, A. (2025): Experimental dataset on the characterization of a new photo-calorespirometry setup Data Brief 63 , art. 112157 10.1016/j.dib.2025.112157 |
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