Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.biortech.2025.133226 |
Licence  |
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| Title (Primary) | Direct real-time determination of photosynthetic efficiency by photo-calorespirometry using Synochocystis sp. PCC 6803 as model cyanobacterium |
| Author | Linke, T.; Paufler, S.; Dusny, C.; Maskow, T.
; Schmid, A. |
| Source Titel | Bioresource Technology |
| Year | 2026 |
| Department | SOMA; MIBITECH |
| Volume | 439 |
| Page From | art. 133226 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | Supplement 1 |
| Keywords | Photosynthesis; Calorimetry; Phototrophic microorganisms; Renewable energy; Oxycaloric equivalent; Photosynthetic adaptation mechanisms |
| Abstract | Photosynthetic efficiency (PE) is key to evaluating phototrophic organisms in biotechnological applications. However, current methods offer limited, indirect insights with poor time resolution. To address this, photo-calorespirometry (Photo-CR) was developed, a novel, non-invasive technique for real-time, direct quantification of photosynthetic energy conversion. Photo-CR simultaneously measures metabolic heat flow and oxygen evolution, capturing both light and dark reactions, including all heat-dissipating side-reactions. Using Synechocystis sp. PCC 6803, it revealed a maximum conserved energy flux of 5.2 ± 0.8W g-1CDW and a light energy yield of 26.4 ± 5.8kJ g-1CDW. A strong correlation between heat and oxygen flux yielded an oxycaloric equivalent of 488.5 ± 0.3kJ mol-1O2 . dynamics, including light saturation and adaptation to fluctuating light, were characterized, showing a maximum PE of 2.3 ± 0.4 % and half-saturation at 360 μmolPhotons m-2 s-1 . Photo-CR integrates thermodynamic and respiratory data, offering novel insights into side-reactions like the Mehler cycle and enabling advanced screening and optimization in photo-biotechnology. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=31288 |
| Linke, T., Paufler, S., Dusny, C., Maskow, T., Schmid, A. (2026): Direct real-time determination of photosynthetic efficiency by photo-calorespirometry using Synochocystis sp. PCC 6803 as model cyanobacterium Bioresour. Technol. 439 , art. 133226 10.1016/j.biortech.2025.133226 |
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