Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.3390/ijms26199314 |
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| Title (Primary) | The endocrine-disrupting chemical benzophenone-3 in concentrations ranging from 0.001 to 10 µM does not affect the human decidualization process in an in vitro setting |
| Author | Krausser, K.; Howanski, J.; Fink, B.; Bauer, M.; Fischer, F.; Romanelli, F.; Zenclussen, A.C.; Schumacher, A. |
| Source Titel | International Journal of Molecular Sciences |
| Year | 2025 |
| Department | IMMU |
| Volume | 26 |
| Issue | 19 |
| Page From | art. 9314 |
| Language | englisch |
| Topic | T9 Healthy Planet |
| Supplements | Supplement 1 |
| Keywords | benzophenone-3; endocrine-disrupting chemicals; decidualization; embryo implantation; trophoblast attachment and invasion |
| Abstract | Endocrine-disrupting chemicals such as benzophenone-3 (BP-3) can have severe consequences for human reproduction by affecting critical processes during pregnancy. To shed further light on potential harmful BP-3 actions, our current study addressed the impact of BP-3 on decidualization and trophoblast invasion. Decidualization was initiated in human endometrial stromal cells (THESC) upon treatment with a mixture of cAMP, progesterone, and estradiol. In parallel to hormonal treatment, the cells were exposed to different BP-3 concentrations ranging from 0.001 µM to 10 µM. The expression of decidualization and invasion markers was determined. Moreover, trophoblastic spheroids derived from JEG-3 cells were transferred to decidualized THESC after BP-3 exposure, and spheroid attachment and invasion were analyzed. Hormonal treatment successfully initiated decidualization in THESC, which was confirmed by increased prolactin levels and IGFBP1 and NCOA-3 mRNA expression. Notably, BP-3 exposure did not affect these markers. Furthermore, BP-3 changed neither THESC proliferation nor viability nor the frequency of cells expressing MMP2/9 or TIMP1/3. Trophoblastic spheroid attachment and outgrowth into THESC were not altered through any of the BP-3 concentrations applied. Our results do not provide evidence for an influence of BP-3 on the decidualization process and the capability of trophoblast cells to adhere and invade into endometrial stromal cells. |
| Krausser, K., Howanski, J., Fink, B., Bauer, M., Fischer, F., Romanelli, F., Zenclussen, A.C., Schumacher, A. (2025): The endocrine-disrupting chemical benzophenone-3 in concentrations ranging from 0.001 to 10 µM does not affect the human decidualization process in an in vitro setting Int. J. Mol. Sci. 26 (19), art. 9314 10.3390/ijms26199314 |
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