Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1002/cssc.202501118 |
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| Title (Primary) | Reducing oxygen stress and improving hydrogen availability boosts microbial electrosynthesis by Clostridium ljungdahlii |
| Author | Kuchenbuch, A.; Al-Sbei, S.; Rosa, L.F.M.; Boto, S.T.; Westermann, M.; Rosenbaum, M.A.; Harnisch, F.
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| Source Titel | ChemSusChem |
| Year | 2025 |
| Department | MIBITECH |
| Page From | art. 2501118 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | https://chemistry-europe.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcssc.202501118&file=cssc70113-sup-0001-SuppData-S1.zip |
| Keywords | bioprocess engineering; Clostridium ljungdahlii; electrobioreactors; green chemistry; microbial electrosynthesis |
| Abstract | Microbial electrosynthesis (MES) holds great promise for converting carbon dioxide (CO2) into building blocks of the (bio)chemical industry. Its advancement is hindered by limited process control and an incomplete understanding of the oxygen (O2) stress response of biocatalysts or key engineering parameters like the availability of hydrogen (H2). With Clostridium ljungdahlii as a model acetogen for strict anaerobic MES from CO2, the effect of O2 stress and H2 availability using 1-L electrobioreactors is showcased, providing high process control and relevance for follow-up engineering and scaling. Using a combinatorial approach of two cathode materials, three anode types, and various current regimes ranging from −5 to −80 mA, MES performance is boosted by overcoming O2 stress and insufficient H2 distribution at high current. It is demonstrated that a large-surface-area carbon fiber fabric cathode combined with O2 evolution anodes flushed with nitrogen (N2) allows the highest reproducible acetate concentration of 12.44 ± 1.56 g L−1 and maximum acetate production rate of 0.6 ± 0.1 g L−1 d−1 reported for MES from CO2 using a pure culture. There is certainly room for improved process control at this and even larger scales, showing that the ceiling of strict anaerobic MES is far from being reached. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=31229 |
| Kuchenbuch, A., Al-Sbei, S., Rosa, L.F.M., Boto, S.T., Westermann, M., Rosenbaum, M.A., Harnisch, F. (2025): Reducing oxygen stress and improving hydrogen availability boosts microbial electrosynthesis by Clostridium ljungdahlii ChemSusChem , art. 2501118 10.1002/cssc.202501118 |
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