Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1002/imt2.70031
Licence creative commons licence
Title (Primary) The microbiologist's guide to metaproteomics
Author Van Den Bossche, T.; Armengaud, J.; Benndorf, D.; Blakeley-Ruiz, J.A.; Brauer, M.; Cheng, K.; Creskey, M.; Figeys, D.; Grenga, L.; Griffin, T.J.; Henry, C.; Hettich, R.L.; Holstein, T.; Jagtap, P.D.; Jehmlich, N. ORCID logo ; Kim, J.; Kleiner, M.; Kunath, B.J.; Malliet, X.; Martens, L.; Mehta, S.; Mesuere, B.; Ning, Z.; Tanca, A.; Uzzau, S.; Verschaffelt, P.; Wang, J.; Wilmes, P.; Zhang, X.; Zhang, X.; Li, L.; The Metaproteomics Initiative,
Source Titel iMeta
Year 2025
Department MOLTOX
Language englisch
Topic T9 Healthy Planet
Supplements https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fimt2.70031&file=imt270031-sup-0001-Supplementary_Information.docx
Keywords bioinformatics; functional dynamics; mass spectrometry; metaproteomics; microbiome
Abstract Metaproteomics is an emerging approach for studying microbiomes, offering the ability to characterize proteins that underpin microbial functionality within diverse ecosystems. As the primary catalytic and structural components of microbiomes, proteins provide unique insights into the active processes and ecological roles of microbial communities. By integrating metaproteomics with other omics disciplines, researchers can gain a comprehensive understanding of microbial ecology, interactions, and functional dynamics. This review, developed by the Metaproteomics Initiative (www.metaproteomics.org), serves as a practical guide for both microbiome and proteomics researchers, presenting key principles, state-of-the-art methodologies, and analytical workflows essential to metaproteomics. Topics covered include experimental design, sample preparation, mass spectrometry techniques, data analysis strategies, and statistical approaches.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=30794
Van Den Bossche, T., Armengaud, J., Benndorf, D., Blakeley-Ruiz, J.A., Brauer, M., Cheng, K., Creskey, M., Figeys, D., Grenga, L., Griffin, T.J., Henry, C., Hettich, R.L., Holstein, T., Jagtap, P.D., Jehmlich, N., Kim, J., Kleiner, M., Kunath, B.J., Malliet, X., Martens, L., Mehta, S., Mesuere, B., Ning, Z., Tanca, A., Uzzau, S., Verschaffelt, P., Wang, J., Wilmes, P., Zhang, X., Zhang, X., Li, L., The Metaproteomics Initiative, (2025):
The microbiologist's guide to metaproteomics
iMeta 10.1002/imt2.70031