Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1002/cssc.202402543 |
Licence  |
|
| Title (Primary) | The impact of redox mediators on the electrogenic and physiological properties of Synechocystis sp. PCC 6803 in a biophotovoltaic system |
| Author | Yuan, J.
; Bai, Y.; Lenz, C.; Reilly-Schott, V.
; Schneider, H.; Lai, B.
; Krömer, J.O. |
| Source Titel | ChemSusChem |
| Year | 2025 |
| Department | MIBITECH |
| Page From | e202402543 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | https://chemistry-europe.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcssc.202402543&file=cssc202402543-s1-Supporting_information.pdf |
| Keywords | 1,4-benzoquinone; ferricyanide; metal-organic complex; extracellular electron transfer; DUAL-KLAS-NIR |
| Abstract | Biophotovoltaics (BPV) is a novel biohybrid solution to utilize solar energy potentially at high energy efficiency, by exploiting the water splitting in oxygenic photoautotrophs and electrochemical electron harvest. Unlike model electrogens, known phototrophic microbes benefit from redox mediators for extracting the photosynthetic electrons and transferring them to the external electron sink for further utilization. In this work, three representative mediators, i.e. 1,4-benzoquinone (BQ), [Co(bpy)3]2+(CoBP), and ferricyanide, were chosen and systematically evaluated for their impacts on the microbial physiology and electrogenic activity of Synechocystis sp. PCC6803. We aimed to generate a knowledge base to guide future mediator selection and design. Our results suggest that ferricyanide remains the best option, as being the only mediator that promoted long-term current output. However, both BQ and CoBP produced higher current densities than ferricyanide, albeit only for a short time. Comprehensive analysis of the photosystem using fluorometric methods suggested that BQ strongly increased the PQ/PQH2 ratio, while CoBP inhibited the electron flow from plastoquinone to photosystem I at high concentrations. Both mediators interrupted the photosynthetic electron flow and consequently cell growth. Eliminating the contribution of storage carbon to the intracellular electron flux demonstrated that all three chemicals could extract electrons originating from water splitting. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=30697 |
| Yuan, J., Bai, Y., Lenz, C., Reilly-Schott, V., Schneider, H., Lai, B., Krömer, J.O. (2025): The impact of redox mediators on the electrogenic and physiological properties of Synechocystis sp. PCC 6803 in a biophotovoltaic system ChemSusChem , e202402543 10.1002/cssc.202402543 |
|