Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.biortech.2025.132124
Title (Primary) Optimising medium chain carboxylate production in xylan mixed-culture monofermentation
Author Iglesias-Riobó, J.; Bonatelli, M.L.; Machado-Fernández, C.; Mauricio-Iglesias, M.; Carballa, M.
Source Titel Bioresource Technology
Year 2025
Department MIBITECH
Volume 420
Page From art. 132124
Language englisch
Topic T7 Bioeconomy
Supplements https://ars.els-cdn.com/content/image/1-s2.0-S0960852425000902-mmc1.docx
Keywords Chain elongation; Hemicellulose; Caproate; Vitamins; Yeast extract
Abstract This work investigates the optimization of medium-chain carboxylate (MCC) production through xylan mixed-culture monofermentation. The pH screening in batch assays showed that the hydrolysis stage and selectivity towards MCC precursors were optimised at pH 6. Subsequently, a continuous stirred tank reactor (CSTR) and a Sequential Batch Reactor (SBR) were operated at different Hydraulic Retention Times (HRT), revealing that the SBR at HRT 2 days yielded the highest caproic acid since lactic acid availability and chain elongation process were balanced. An enriched medium with yeast extract and vitamins favoured the growth of chain elongators, and therefore, the MCC production. Moreover, cross-feeding interaction between bacteria in xylan fermentation was observed, and Pseudoramibacter was present in the highest caproic acid yields. This work highlights the impact of selecting the proper operational window to optimise one-stage MCC production.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=30382
Iglesias-Riobó, J., Bonatelli, M.L., Machado-Fernández, C., Mauricio-Iglesias, M., Carballa, M. (2025):
Optimising medium chain carboxylate production in xylan mixed-culture monofermentation
Bioresour. Technol. 420 , art. 132124 10.1016/j.biortech.2025.132124