Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.chemosphere.2024.143503 |
| Title (Primary) | Integrated induction of silver resistance determinants and production of extracellular polymeric substances in Cupriavidus metallidurans BS1 in response to silver ions and silver nanoparticles |
| Author | Dai, J.-Y.; Yu, Y.; You, L.-X.; Zhong, H.-L.; Li, Y.-P.; Wang, A.-J.; Chorover, J.; Feng, R.-W.; Alwathnani, H.A.; Herzberg, M.
; Rensing, C. |
| Source Titel | Chemosphere |
| Year | 2024 |
| Department | SOMA |
| Volume | 366 |
| Page From | art. 143503 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | https://ars.els-cdn.com/content/image/1-s2.0-S0045653524024032-mmc1.pdf |
| Keywords | Silver-resistance; Cupriavidus metallidurans; Extracellular polymeric substances; Silver nanoparticle |
| Abstract | Although the antimicrobial mechanisms of nanomaterials have been
extensively investigated, bacterial defense mechanisms associated with
AgNPs have not been fully elucidated. We here report that dissolved Ag+ (>0.05 μg mL−1) displayed higher toxicity on cell growth of strain Cupriavidus metallidurans
BS1 (GCA_003260185.2) in comparison to 2 and 20 nm AgNPs. The genes
necessary for synthesis of distinct abundance and composition of
extracellular polymeric substances (EPS) were induced in strain BS1
exposed to Ag stress. This resulted in 20.1% (Ag(I)-EPS) and 24.2% (2 nm
AgNPs-EPS) of the C O
band integrated intensities being converted into C-OH/C-O-C group
vibrations and the Ag-O bond was formed between EPS and 20 nm AgNPs.
Meanwhile, the expression of primary resistance genes of the cus, sil and cup operon encoding HME-RND-driven efflux systems as well as a PIB1-type
ATPase (CupA) were significantly induced after exposure to Ag(I), 2 and
20 nm AgNPs, respectively. Furthermore, distinct genes involved in
biosynthesis pathways responsible for production of EPS were induced to
relieve the toxicity of Ag(I), 2 nm and 20 nm AgNPs. This combined
action is one potential reason why strain BS1 displayed distinct
resistances in response to Ag(I) compared to 2 and 20 nm AgNPs. This
work will help in understanding processes important in bacterial
defensive mechanisms to AgNPs. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=29847 |
| Dai, J.-Y., Yu, Y., You, L.-X., Zhong, H.-L., Li, Y.-P., Wang, A.-J., Chorover, J., Feng, R.-W., Alwathnani, H.A., Herzberg, M., Rensing, C. (2024): Integrated induction of silver resistance determinants and production of extracellular polymeric substances in Cupriavidus metallidurans BS1 in response to silver ions and silver nanoparticles Chemosphere 366 , art. 143503 10.1016/j.chemosphere.2024.143503 |
|