Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.envint.2024.108732
Licence creative commons licence
Title (Primary) Priority establishment of soil bacteria in rhizosphere limited the spread of tetracycline resistance genes from pig manure to soil-plant systems based on synthetic communities approach
Author Wen, X.; Xu, J.; Worrich, A.; Li, X.; Yuan, X.; Ma, B.; Zou, Y.; Wang, Y.; Liao, X.; Wu, Y.
Source Titel Environment International
Year 2024
Department AME
Volume 187
Page From art. 108732
Language englisch
Topic T7 Bioeconomy
Supplements https://ars.els-cdn.com/content/image/1-s2.0-S0160412024003180-mmc1.docx
Keywords Synthetic communities; Antibiotic resistance genes; Pig manure; Soil bacteria; Doxycycline; Soil–plant system
Abstract The spread of antibiotic resistance genes (ARGs) in agroecosystems through the application of animal manure is a global threat to human and environmental health. However, the adaptability and colonization ability of animal manure-derived bacteria determine the spread pathways of ARG in agroecosystems, which have rarely been studied. Here, we performed an invasion experiment by creating a synthetic communities (SynCom) with ten isolates from pig manure and followed its assembly during gnotobiotic cultivation of a soil-Arabidopsis thaliana (A. thaliana) system. We found that Firmicutes in the SynCom were efficiently filtered out in the rhizosphere, thereby limiting the entry of tetracycline resistance genes (TRGs) into the plant. However, Proteobacteria and Actinobacteria in the SynCom were able to establish in all compartments of the soil–plant system thereby spreading TRGs from manure to soil and plant. The presence of native soil bacteria prevented the establishment of manure-borne bacteria and effectively reduced the spread of TRGs. Achromobacter mucicolens and Pantoea septica were the main vectors for the entry of tetA into plants. Furthermore, doxycycline stress promoted the horizontal gene transfer (HGT) of the conjugative resistance plasmid RP4 within the SynCom in A. thaliana by upregulating the expression of HGT-related mRNAs. Therefore, this study provides evidence for the dissemination pathways of ARGs in agricultural systems through the invasion of manure-derived bacteria and HGT by conjugative resistance plasmids and demonstrates that the priority establishment of soil bacteria in the rhizosphere limited the spread of TRGs from pig manure to soil-plant systems.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=29256
Wen, X., Xu, J., Worrich, A., Li, X., Yuan, X., Ma, B., Zou, Y., Wang, Y., Liao, X., Wu, Y. (2024):
Priority establishment of soil bacteria in rhizosphere limited the spread of tetracycline resistance genes from pig manure to soil-plant systems based on synthetic communities approach
Environ. Int. 187 , art. 108732 10.1016/j.envint.2024.108732