Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.ecoenv.2024.116425 |
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| Title (Primary) | Biosynthesis of silver nanoparticles using Burkholderia contaminans ZCC and mechanistic analysis at the proteome level |
| Author | You, L.-X.; Zhong, H.-L.; Chen, S.-R.; Sun, Y.-N.; Wu, G.-K.; Zhao, M.-Z.; Hu, S.-S.; Alwathnani, H.; Herzberg, M.
; Qin, S.-F.; Rensing, C. |
| Source Titel | Ecotoxicology and Environmental Safety |
| Year | 2024 |
| Department | EAC |
| Volume | 278 |
| Page From | art. 116425 |
| Language | englisch |
| Topic | T9 Healthy Planet |
| Supplements | https://ars.els-cdn.com/content/image/1-s2.0-S0147651324005013-mmc1.doc |
| Keywords | Silver nanoparticles; Burkholderia contaminans ZCC; Extracellular electron transfer; Biosynthesis; Proteomic analysis |
| Abstract | The biogenic synthesis of silver nanoparticles (AgNPs) by microorganisms
has been a subject of increasing attention. Despite extensive studies
on this biosynthetic pathway, the mechanisms underlying the involvement
of proteins and enzymes in AgNPs production have not been fully
explored. Herein, we reported that Burkholderia contaminans ZCC was able to reduce Ag+ to AgNPs with a diameter of (10±5) nm inside the cell. Exposure of B. contaminans ZCC to Ag+
ions led to significant changes in the functional groups of cellular
proteins, with approximately 5.72% of the (C-OH) bonds being converted
to (C-C/C-H) (3.61%) and C O (2.11%) bonds, and 4.52% of the CO (carbonyl) bonds being converted to (C-OH) bonds. Furthermore, the presence of Ag+
and AgNPs induced the ability of extracellular electron transfer for
ZCC cells via specific membrane proteins, but this did not occur in the
absence of Ag+ ions. Proteomic analysis of the proteins and
enzymes involved in heavy metal efflux systems, protein secretion
system, oxidative phosphorylation, intracellular electron transfer
chain, and glutathione metabolism suggests that glutathione
S-transferase and ubiquinol-cytochrome c reductase iron-sulfur
subunit play importance roles in the biosynthesis of AgNPs. These
findings contribute to a deeper understanding of the functions exerted
by glutathione S-transferase and ferredoxin-thioredoxin reductase
iron-sulfur subunits in the biogenesis of AgNPs, thereby hold immense
potential for optimizing biotechnological techniques aimed at enhancing
the yield and purity of biosynthetic AgNPs. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=29233 |
| You, L.-X., Zhong, H.-L., Chen, S.-R., Sun, Y.-N., Wu, G.-K., Zhao, M.-Z., Hu, S.-S., Alwathnani, H., Herzberg, M., Qin, S.-F., Rensing, C. (2024): Biosynthesis of silver nanoparticles using Burkholderia contaminans ZCC and mechanistic analysis at the proteome level Ecotox. Environ. Safe. 278 , art. 116425 10.1016/j.ecoenv.2024.116425 |
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