Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1093/ismejo/wrae086
Licence creative commons licence
Title (Primary) Metabolic potential of Nitrososphaera-associated clades
Author Bei, Q. ORCID logo ; Reitz, T.; Schädler, M.; Hodgskiss, L.H.; Peng, J.; Schnabel, B.; Buscot, F.; Eisenhauer, N.; Schleper, C.; Heintz-Buschart, A.
Source Titel The ISME Journal
Year 2024
Department BZF; BOOEK; iDiv
Volume 18
Issue 1
Page From wrae086
Language englisch
Topic T5 Future Landscapes
Data and Software links
Keywords ammonia-oxidizing archaea; Nitrososphaerales; 54d9, metagenomics
Abstract Soil ammonia-oxidizing archaea (AOA) play a crucial role in converting ammonia to nitrite, thereby mobilizing reactive nitrogen species into their soluble form, with a significant impact on nitrogen losses from terrestrial soils. Yet, our knowledge regarding their diversity and functions remains limited. In this study, we reconstructed 97 high-quality AOA metagenome-assembled genomes (MAGs) from 180 soil samples collected in Central Germany during 2014–2019 summers. These MAGs were affiliated with the order Nitrososphaerales and clustered into four family-level clades (NS-α/γ/δ/ε). Among these MAGs, 75 belonged to the most abundant but least understood δ-clade. Within the δ-clade, the amoA genes in three MAGs from neutral soils showed a 99.5% similarity to the fosmid clone 54d9, which has served as representative of the δ-clade for the past two decades since even today no cultivated representatives are available. Seventy-two MAGs constituted a distinct δ sub-clade, and their abundance and expression activity were more than twice that of other MAGs in slightly acidic soils. Unlike the less abundant clades (α, γ, and ε), the δ-MAGs possessed multiple highly expressed intracellular and extracellular carbohydrate-active enzymes responsible for carbohydrate binding (CBM32) and degradation (GH5), along with highly expressed genes involved in ammonia oxidation. Together, these results suggest metabolic versatility of uncultured soil AOA and a potential mixotrophic or chemolithoheterotrophic lifestyle among 54d9-like AOA.
Persistent UFZ Identifier
Bei, Q., Reitz, T., Schädler, M., Hodgskiss, L.H., Peng, J., Schnabel, B., Buscot, F., Eisenhauer, N., Schleper, C., Heintz-Buschart, A. (2024):
Metabolic potential of Nitrososphaera-associated clades
ISME J. 18 (1), wrae086 10.1093/ismejo/wrae086