Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1038/s42003-024-06204-7
Licence creative commons licence
Title (Primary) Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts
Author Schmidt, L.; Tüting, C.; Kyrilis, F.L.; Hamdi, F.; Semchonok, D.A.; Hause, G.; Meister, A.; Ihling, C.; Stubbs, M.T.; Sinz, A.; Kastritis, P.L.
Source Titel Communications Biology
Year 2024
Department TECH
Volume 7
Page From art. 557
Language englisch
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Abstract The high abundance of most viruses in infected host cells benefits their structural characterization. However, endogenous viruses are present in low copy numbers and are therefore challenging to investigate. Here, we retrieve cell extracts enriched with an endogenous virus, the yeast L-A virus. The determined cryo-EM structure discloses capsid-stabilizing cation-π stacking, widespread across viruses and within the Totiviridae, and an interplay of non-covalent interactions from ten distinct capsomere interfaces. The capsid-embedded mRNA decapping active site trench is supported by a constricting movement of two flexible opposite-facing loops. tRNA-loaded polysomes and other biomacromolecules, presumably mRNA, are found in virus proximity within the cell extract. Mature viruses participate in larger viral communities resembling their rare in-cell equivalents in terms of size, composition, and inter-virus distances. Our results collectively describe a 3D-architecture of a viral milieu, opening the door to cell-extract-based high-resolution structural virology.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=29134
Schmidt, L., Tüting, C., Kyrilis, F.L., Hamdi, F., Semchonok, D.A., Hause, G., Meister, A., Ihling, C., Stubbs, M.T., Sinz, A., Kastritis, P.L. (2024):
Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts
Commun. Biol. 7 , art. 557 10.1038/s42003-024-06204-7