Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1038/s41598-023-49505-3
Licence creative commons licence
Title (Primary) Changes of the bacterial composition in duodenal fluid from patients with liver cirrhosis and molecular bacterascites
Author Höppner, J.; Krohn, S.; van den Munckhof, E.H.A.; Kallies, R.; Herber, A.; Zeller, K.; Tünnemann, J.; Matz-Soja, M.; Chatzinotas, A.; Böhm, S.; Hoffmeister, A.; Berg, T.; Engelmann, C.
Source Titel Scientific Reports
Year 2023
Department UMB
Volume 13
Page From art. 23001
Language englisch
Topic T7 Bioeconomy
Abstract Small intestinal bacterial overgrowth and compositional changes of intestinal microbiota are pathomechanistic factors in liver cirrhosis leading to bacterial translocation and infectious complications. We analyzed the quantity and composition of duodenal bacterial DNA (bactDNA) in relation to bactDNA in blood and ascites of patients with liver cirrhosis. Duodenal fluid and corresponding blood and ascites samples from 103 patients with liver cirrhosis were collected. Non-liver disease patients (n = 22) served as controls. BactDNA was quantified by 16S-rRNA gene-based PCR. T-RFLP and 16S-rRNA amplicon sequencing were used to analyze bacterial composition. Duodenal bacterial diversity in cirrhosis was distinct to controls showing significantly higher abundances of Streptococcus, Enterococcus and Veillonella. Patients with bactDNA positive ascites revealed reduced spectrum of core microbiota with Streptococcus as key player of duodenal community and higher prevalence of Granulicatella proving presence of cirrhosis related intestinal dysbiosis. Regarding duodenal fluid bactDNA quantification, no significant differences were found between patients with cirrhosis and controls. Additionally, percentage of subjects with detectable bactDNA in blood did not differ between patients and controls. This study evaluated the diversity of bacterial DNA in different body specimens with potential implications on understanding how intestinal bacterial translocation may affect infectious complications in cirrhosis.
Persistent UFZ Identifier
Höppner, J., Krohn, S., van den Munckhof, E.H.A., Kallies, R., Herber, A., Zeller, K., Tünnemann, J., Matz-Soja, M., Chatzinotas, A., Böhm, S., Hoffmeister, A., Berg, T., Engelmann, C. (2023):
Changes of the bacterial composition in duodenal fluid from patients with liver cirrhosis and molecular bacterascites
Sci. Rep. 13 , art. 23001 10.1038/s41598-023-49505-3