Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1038/s41467-023-43448-z
Licence creative commons licence
Title (Primary) Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting
Author Riva, A.; Rasoulimehrabani, H.; Cruz-Rubio, J.M.; Schnorr, S.L.; von Baeckmann, C.; Inan, D.; Nikolov, G.; Herbold, C.W.; Hausmann, B.; Pjevac, P.; Schintlmeister, A.; Spittler, A.; Palatinszky, M.; Kadunic, A.; Hieger, N.; Del Favero, G.; von Bergen, M.; Jehmlich, N. ORCID logo ; Watzka, M.; Lee, K.S.; Wiesenbauer, J.; Khadem, S.; Viernstein, H.; Stocker, R.; Wagner, M.; Kaiser, C.; Richter, A.; Kleitz, F.; Berry, D.
Source Titel Nature Communications
Year 2023
Department MOLSYB
Volume 14
Page From art. 8210
Language englisch
Topic T9 Healthy Planet
Abstract Prebiotics are defined as non-digestible dietary components that promote the growth of beneficial gut microorganisms. In many cases, however, this capability is not systematically evaluated. Here, we develop a methodology for determining prebiotic-responsive bacteria using the popular dietary supplement inulin. We first identify microbes with a capacity to bind inulin using mesoporous silica nanoparticles functionalized with inulin. 16S rRNA gene amplicon sequencing of sorted cells revealed that the ability to bind inulin was widespread in the microbiota. We further evaluate which taxa are metabolically stimulated by inulin and find that diverse taxa from the phyla Firmicutes and Actinobacteria respond to inulin, and several isolates of these taxa can degrade inulin. Incubation with another prebiotic, xylooligosaccharides (XOS), in contrast, shows a more robust bifidogenic effect. Interestingly, the Coriobacteriia Eggerthella lenta and Gordonibacter urolithinfaciens are indirectly stimulated by the inulin degradation process, expanding our knowledge of inulin-responsive bacteria.
Persistent UFZ Identifier
Riva, A., Rasoulimehrabani, H., Cruz-Rubio, J.M., Schnorr, S.L., von Baeckmann, C., Inan, D., Nikolov, G., Herbold, C.W., Hausmann, B., Pjevac, P., Schintlmeister, A., Spittler, A., Palatinszky, M., Kadunic, A., Hieger, N., Del Favero, G., von Bergen, M., Jehmlich, N., Watzka, M., Lee, K.S., Wiesenbauer, J., Khadem, S., Viernstein, H., Stocker, R., Wagner, M., Kaiser, C., Richter, A., Kleitz, F., Berry, D. (2023):
Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting
Nat. Commun. 14 , art. 8210 10.1038/s41467-023-43448-z