Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.envint.2024.108426
Licence creative commons licence
Title (Primary) Assessing background contamination of sample tubes used in human biomonitoring by non-targeted liquid chromatography–high resolution mass spectrometry
Author Krauss, M. ORCID logo ; Huber, C.; Schulze, T. ORCID logo ; Bartel-Steinbach, M.; Weber, T.; Kolossa-Gehring, M.; Lermen, D.
Source Titel Environment International
Year 2024
Department EXPO
Volume 138
Page From art. 108426
Language englisch
Topic T9 Healthy Planet
Data and Software links
Keywords Non-target-screening; Human biomonitoring; Quality control; Background contamination; Sample tubes
Abstract Controlling and minimising background contamination is crucial for maintaining a high quality of samples in human biomonitoring targeting organic chemicals. We assessed the contamination of three previous types and one newly introduced medical-grade type of sample tubes used for storing human body fluids at the German Environmental Specimen Bank. Aqueous extracts from these tubes were analysed by non-targeted liquid chromatography-high resolution mass spectrometry (LC-HRMS) before and after a dedicated cleaning procedure. After peak detection using MZmine, Bayesian hypothesis testing was used to group peaks into those originating either from instrumental and laboratory background contamination, or actual tube contaminants, based on if their peak height was reduced, increased or not affected by the cleaning procedure. For all four tube types 80–90% of the 2475 peaks (1549 in positive and 926 in negative mode) were assigned to laboratory/instrumental background, which we have to consider as potential sample tube contaminants. Among the tube contaminants, results suggest a considerable difference in the contaminant peak inventory and the absolute level of contamination among the different sample tube types. The cleaning procedure did not affect the largest fraction of peaks (50–70%). For the medical grade tubes, the removal of contaminants by the cleaning procedure was strongest compared to the previous tubes, but in all cases a small fraction increased in intensity after cleaning, probably due to a release of oligomers or additives. The identified laboratory background contaminants were mainly semi-volatile polymer additives such as phthalates and phosphate esters. A few compounds could be assigned solely as tube-specific contaminants, such as N,N-dibutylformamide and several constituents of the oligomeric light stabiliser Tinuvin-622. A cleaning procedure before use is an effective way to standardise the used sample tubes and minimises the background contamination, and therefore increases sample quality and therewith analytical results.
Persistent UFZ Identifier
Krauss, M., Huber, C., Schulze, T., Bartel-Steinbach, M., Weber, T., Kolossa-Gehring, M., Lermen, D. (2024):
Assessing background contamination of sample tubes used in human biomonitoring by non-targeted liquid chromatography–high resolution mass spectrometry
Environ. Int. 138 , art. 108426 10.1016/j.envint.2024.108426