Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.scitotenv.2023.168689
Title (Primary) Active soil microbial composition and proliferation are directly affected by the presence of biocides from building materials
Author Reiß, F.; Kiefer, N.; Purahong, W.; Borken, W.; Kalkhof, S.; Noll, M.
Source Titel Science of the Total Environment
Year 2024
Department BOOEK
Volume 912
Page From art. 168689
Language englisch
Topic T5 Future Landscapes
Keywords Bacteria; Biocides; Bromodeoxyuridine; Fungi; Soil microbiome; Terrestrial ecotoxicological effects
Abstract Combinations of biocides are commonly added to building materials to prevent microbial growth and thereby cause degradation of the façades. These biocides reach the environment by leaching from façades posing an environmental risk. Although ecotoxicity to the aquatic habitat is well established, there is hardly any data on the ecotoxicological effects of biocides on the soil habitat. This study aimed to characterize the effect of the biocides terbutryn, isoproturon, octhilinone, and combinations thereof on the total and active soil microbial community composition and functions. Total soil microbial community was retrieved directly from the nucleic acid extracts, while the DNA of the active soil microbial community was separated after bromodeoxyuridine labeling. Bacterial 16S rRNA gene and fungal transcribed spacer region gene-based amplicon sequencing was carried out for both active and total, while gene copy numbers were quantified only for the total soil microbial community. Additionally, soil respiration and physico-chemical parameters were analyzed to investigate overall soil microbial activity. The bacterial and fungal gene copy numbers were significantly affected by single biocides and combined biocide soil treatment but not soil respiration and physico-chemical parameters. Moreover, results showed that single and combined biocide treatment only had minor effects on the total soil microbiome. While the total soil microbiome experienced only minor effects from single and combined biocide treatment, the active soil microbiome was significantly impacted in its diversity, richness, composition, and functional patterns. The active bacterial richness was more sensitive than fungi. However, the adverse effects of the biocide combination treatments on soil bacterial richness were highly dependent on the identities of the biocide combination. Our results demonstrate that the presence of biocides frequently used in building materials affects the active soil microbiome. Thereby, the approach described herein can be used as an ecotoxicological measure for the effect on complex soil environments in future studies.
Persistent UFZ Identifier
Reiß, F., Kiefer, N., Purahong, W., Borken, W., Kalkhof, S., Noll, M. (2024):
Active soil microbial composition and proliferation are directly affected by the presence of biocides from building materials
Sci. Total Environ. 912 , art. 168689 10.1016/j.scitotenv.2023.168689