Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1021/acs.est.3c05932
Title (Primary) Improve niche colonization and microbial interactions for organohalide-respiring-bacteria-mediated remediation of chloroethene-contaminated sites
Author Wu, R.; Shen, R.; Liang, Z.; Zheng, S.; Yang, Y.; Lu, Q.; Adrian, L.; Wang, S.
Source Titel Environmental Science & Technology
Year 2023
Department UBT
Volume 57
Issue 45
Page From 17338
Page To 17352
Language englisch
Topic T7 Bioeconomy
Keywords bioremediation; microbial reductive dechlorination; chloroethenes; colonization; organohalide-respiring bacteria; Dehalococcoides; Geobacter
Abstract Organohalide-respiring bacteria (OHRB)-mediated reductive dehalogenation is promising in in situ bioremediation of chloroethene-contaminated sites. The bioremediation efficiency of this approach is largely determined by the successful colonization of fastidious OHRB, which is highly dependent on the presence of proper growth niches and microbial interactions. In this study, based on two ecological principles (i.e., Priority Effects and Coexistence Theory), three strategies were developed to enhance niche colonization of OHRB, which were tested both in laboratory experiments and field applications: (i) preinoculation of a niche-preparing culture (NPC, being mainly constituted of fermenting bacteria and methanogens); (ii) staggered fermentation; and (iii) increased inoculation of CE40 (a Dehalococcoides-containing tetrachloroethene-to-ethene dechlorinating enrichment culture). Batch experimental results show significantly higher dechlorination efficiencies, as well as lower concentrations of volatile fatty acids (VFAs) and methane, in experimental sets with staggered fermentation and niche-preconditioning with NPC for 4 days (CE40_NPC-4) relative to control sets. Accordingly, a comparatively higher abundance of Dehalococcoides as major OHRB, together with a lower abundance of fermenting bacteria and methanogens, was observed in CE40_NPC-4 with staggered fermentation, which indicated the balanced syntrophic and competitive interactions between OHRB and other populations for the efficient dechlorination. Further experiments with microbial source tracking analyses suggested enhanced colonization of OHRB by increasing the inoculation ratio of CE40. The optimized conditions for enhanced colonization of OHRB were successfully employed for field bioremediation of trichloroethene (TCE, 0.3–1.4 mM)- and vinyl chloride (VC, ∼0.04 mM)-contaminated sites, resulting in 96.6% TCE and 99.7% VC dechlorination to ethene within 5 and 3 months, respectively. This study provides ecological principles-guided strategies for efficient bioremediation of chloroethene-contaminated sites, which may be also employed for removal of other emerging organohalide pollutants.
Persistent UFZ Identifier
Wu, R., Shen, R., Liang, Z., Zheng, S., Yang, Y., Lu, Q., Adrian, L., Wang, S. (2023):
Improve niche colonization and microbial interactions for organohalide-respiring-bacteria-mediated remediation of chloroethene-contaminated sites
Environ. Sci. Technol. 57 (45), 17338 - 17352 10.1021/acs.est.3c05932