Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.csbj.2023.10.046
Licence creative commons licence
Title (Primary) Structure-function and engineering of plant UDP-glycosyltransferase
Author Wang, M.; Ji, Q.; Lai, B. ORCID logo ; Liu, Y.; Mei, K.
Source Titel Computational and Structural Biotechnology Journal
Year 2023
Department UMB
Volume 21
Page From 5358
Page To 5371
Language englisch
Topic T7 Bioeconomy
Keywords Glycosyltransferase; UGT; Structure; Mechanism; Protein engineering
Abstract Natural products synthesized by plants have substantial industrial and medicinal values and are therefore attracting increasing interest in various related industries. Among the key enzyme families involved in the biosynthesis of natural products, uridine diphosphate-dependent glycosyltransferases (UGTs) play a crucial role in plants. In recent years, significant efforts have been made to elucidate the catalytic mechanisms and substrate recognition of plant UGTs and to improve them for desired functions. In this review, we presented a comprehensive overview of all currently published structures of plant UGTs, along with in-depth analyses of the corresponding catalytic and substrate recognition mechanisms. In addition, we summarized and evaluated the protein engineering strategies applied to improve the catalytic activities of plant UGTs, with a particular focus on high-throughput screening methods. The primary objective of this review is to provide readers with a comprehensive understanding of plant UGTs and to serve as a valuable reference for the latest techniques used to improve their activities.
Persistent UFZ Identifier
Wang, M., Ji, Q., Lai, B., Liu, Y., Mei, K. (2023):
Structure-function and engineering of plant UDP-glycosyltransferase
Comp. Struct. Biotechnol. J. 21 , 5358 - 5371 10.1016/j.csbj.2023.10.046