Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.15698/mic2023.09.804 |
Licence  |
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| Title (Primary) | Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2 |
| Author | Arend, M.; Ütkür, K.; Hawer, H.; Mayer, K.; Ranjan, N.; Adrian, L.
; Brinkmann, U.; Schaffrath, R. |
| Source Titel | Microbial Cell |
| Year | 2023 |
| Department | UBT |
| Volume | 10 |
| Issue | 9 |
| Page From | 195 |
| Page To | 203 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | http://microbialcell.com/wordpress/wp-content/uploads/2023A-Arend-Microbial-Cell-Supplementals.pdf |
| Keywords | budding yeast; EF2 diphthamide modification; diphtheria toxin; tRNA modification; elongator; tRNase zymocin |
| Abstract | In yeast, Elongator-dependent tRNA modifications are regulated by the Kti11•Kti13 dimer and hijacked for cell killing by zymocin, a tRNase ribotoxin. Kti11 (alias Dph3) also controls modification of elongation factor 2 (EF2) with diphthamide, the target for lethal ADP-ribosylation by diphtheria toxin (DT). Diphthamide formation on EF2 involves four biosynthetic steps encoded by the DPH1-DPH7 network and an ill-defined KTI13 function. On further examining the latter gene in yeast, we found that kti13Δ null-mutants maintain unmodified EF2 able to escape ADP-ribosylation by DT and to survive EF2 inhibition by sordarin, a diphthamide-dependent antifungal. Consistently, mass spectrometry shows kti13Δ cells are blocked in proper formation of amino-carboxyl-propyl-EF2, the first diphthamide pathway intermediate. Thus, apart from their common function in tRNA modification, both Kti11/Dph3 and Kti13 share roles in the initiation step of EF2 modification. We suggest an alias KTI13/DPH8 nomenclature indicating dual-functionality analogous to KTI11/DPH3. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28041 |
| Arend, M., Ütkür, K., Hawer, H., Mayer, K., Ranjan, N., Adrian, L., Brinkmann, U., Schaffrath, R. (2023): Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2 Microb. Cell 10 (9), 195 - 203 10.15698/mic2023.09.804 |
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