Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1038/s41598-023-44256-7
Licence creative commons licence
Title (Primary) Hypertonic stress induced changes of Pseudomonas fluorescens adhesion towards soil minerals studied by AFM
Author Abu Quba, A.A.; Goebel, M.-O.; Karagulyan, M.; Miltner, A. ORCID logo ; Kästner, M.; Bachmann, J.; Schaumann, G.E.; Diehl, D.
Source Titel Scientific Reports
Year 2023
Department UBT
Volume 13
Page From art. 17146
Language englisch
Topic T7 Bioeconomy
Data and Software links https://doi.org/10.17605/OSF.IO/6GUWD
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-023-44256-7/MediaObjects/41598_2023_44256_MOESM1_ESM.pdf
Keywords Biogeochemistry; Environmental sciences
Abstract Studying bacterial adhesion to mineral surfaces is crucial for understanding soil properties. Recent research suggests that minimal coverage of sand particles with cell fragments significantly reduces soil wettability. Using atomic force microscopy (AFM), we investigated the influence of hypertonic stress on Pseudomonas fluorescens adhesion to four different minerals in water. These findings were compared with theoretical XDLVO predictions. To make adhesion force measurements comparable for irregularly shaped particles, we normalized adhesion forces by the respective cell-mineral contact area. Our study revealed an inverse relationship between wettability and the surface-organic carbon content of the minerals. This relationship was evident in the increased adhesion of cells to minerals with decreasing wettability. This phenomenon was attributed to hydrophobic interactions, which appeared to be predominant in all cell–mineral interaction scenarios alongside with hydrogen bonding. Moreover, while montmorillonite and goethite exhibited stronger adhesion to stressed cells, presumably due to enhanced hydrophobic interactions, kaolinite showed an unexpected trend of weaker adhesion to stressed cells. Surprisingly, the adhesion of quartz remained independent of cell stress level. Discrepancies between measured cell–mineral interactions and those calculated by XDLVO, assuming an idealized sphere-plane geometry, helped us interpret the chemical heterogeneity arising from differently exposed edges and planes of minerals. Our results suggest that bacteria may have a significant impact on soil wettability under changing moisture condition.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28034
Abu Quba, A.A., Goebel, M.-O., Karagulyan, M., Miltner, A., Kästner, M., Bachmann, J., Schaumann, G.E., Diehl, D. (2023):
Hypertonic stress induced changes of Pseudomonas fluorescens adhesion towards soil minerals studied by AFM
Sci. Rep. 13 , art. 17146