Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1021/acs.est.3c02819 |
Licence  |
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| Title (Primary) | Uptake and biotransformation of the tire rubber-derived contaminants 6-PPD and 6-PPD quinone in the zebrafish embryo (Danio rerio) |
| Author | Grasse, N.
; Seiwert, B.; Massei, R.; Scholz, S.
; Fu, Q.; Reemtsma, T. |
| Source Titel | Environmental Science & Technology |
| Year | 2023 |
| Department | BIOTOX; ANA; MET |
| Volume | 57 |
| Issue | 41 |
| Page From | 15598 |
| Page To | 15607 |
| Language | englisch |
| Topic | T9 Healthy Planet T5 Future Landscapes |
| Supplements | https://pubs.acs.org/doi/suppl/10.1021/acs.est.3c02819/suppl_file/es3c02819_si_001.pdf https://pubs.acs.org/doi/suppl/10.1021/acs.est.3c02819/suppl_file/es3c02819_si_002.xlsx |
| Keywords | phase II metabolism; tire and road wear particles; suspect and nontarget screening; aquatic organisms; water quality |
| Abstract | N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6-PPD) is a widely used antioxidant in tire rubber known to enter the aquatic environment via road runoff. The associated transformation product (TP) 6-PPD quinone (6-PPDQ) causes extreme acute toxicity in some fish species (e.g., coho salmon). To interpret the species-specific toxicity, information about biotransformation products of 6-PPDQ would be relevant. This study investigated toxicokinetics of 6-PPD and 6-PPDQ in the zebrafish embryo (ZFE) model. Over 96 h of exposure, 6-PPD and 6-PPDQ accumulated in the ZFE with concentration factors ranging from 140 to 2500 for 6-PPD and 70 to 220 for 6-PPDQ. A total of 22 TPs of 6-PPD and 12 TPs of 6-PPDQ were tentatively identified using liquid chromatography coupled to high-resolution mass spectrometry. After 96 h of exposure to 6-PPD, the TPs of 6-PPD comprised 47% of the total peak area (TPA), with 4-hydroxydiphenylamine being the most prominent in the ZFE. Upon 6-PPDQ exposure, >95% of 6-PPDQ taken up in the ZFE was biotransformed, with 6-PPDQ + O + glucuronide dominating (>80% of the TPA). Among other TPs of 6-PPD, a reactive N-phenyl-p-benzoquinone imine was found. The knowledge of TPs of 6-PPD and 6-PPDQ from this study may support biotransformation studies in other organisms. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=27906 |
| Grasse, N., Seiwert, B., Massei, R., Scholz, S., Fu, Q., Reemtsma, T. (2023): Uptake and biotransformation of the tire rubber-derived contaminants 6-PPD and 6-PPD quinone in the zebrafish embryo (Danio rerio) Environ. Sci. Technol. 57 (41), 15598 - 15607 10.1021/acs.est.3c02819 |
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