Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1021/ac950692n
Title (Primary) A nitrite sensor based on a highly sensitive nitrite reductase mediator-coupled amperometric detection
Author Strehlitz, B.; Gründig, B.; Schumacher, W.; Kroneck, P.M.H.; Vorlop, K.-D.; Kotte, H.
Source Titel Analytical Chemistry
Year 1996
Department UBZ; SAN
Volume 68
Issue 5
Page From 807
Page To 816
Language englisch
Abstract Highly sensitive nitrite sensors have been developed for the first time based on mediator-modified electrodes. Tetraheme cytochrome c nitrite reductase from Sulfurospirillum deleyianum and cytochrome cd1 nitrite reductase from Paracoccus denitrificans are able to accept electrons from artificial electron donors, which simultaneously act as electron mediators between the enzyme and an amperometric electrode. In addition to methyl viologen, redox-active compounds such as phenazines (phenosafranin, safranin T, N-methylphenazinium, 1-methoxy-N-methylphenazinium) and triarylmethane redox dyes (bromphenol blue and red) were selected from a range of redox compounds exhibiting the most efficient performance for nitrite detection. After precipitation, the electron mediators were incorporated in a graphite electrode material. Enzyme immobilization is performed by entrapment in a poly(carbamoyl sulfonate) (PCS) hydrogel. Diffusion coefficients and apparent heterogeneous rate constants of the mediators as well as homogeneous rate constants of nitrite sensors were determined by chronoamperometry and cyclic voltammetry. The phenosafranin-modified electrode layered with the PCS hydrogel immobilization of tetraheme cytochrome c nitrite reductase yielded linear current responses up to 250 μM nitrite with a sensitivity of 446.5 mA M-1 cm-2. The detection limit of the enzymatic nitrite sensor was found to be 1 μM nitrite.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=27807
Strehlitz, B., Gründig, B., Schumacher, W., Kroneck, P.M.H., Vorlop, K.-D., Kotte, H. (1996):
A nitrite sensor based on a highly sensitive nitrite reductase mediator-coupled amperometric detection
Anal. Chem. 68 (5), 807 - 816 10.1021/ac950692n