Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.copbio.2023.102977
Title (Primary) Microbial single-cell mass spectrometry: status, challenges, and prospects
Author Schirmer, M.; Dusny, C.
Source Titel Current Opinion in Biotechnology
Year 2023
Department SOMA
Volume 83
Page From art. 102977
Language englisch
Topic T7 Bioeconomy

Single-cell analysis uncovers phenotypic differences between cells in a population and dissects their individual physiological states and differences on all omics levels from genome to phenome. Spectrometric observation allows label-free analysis of the metabolome and proteome of individual cells, but is still mainly limited to the analysis of mammalian single cells. Recent progress in mass spectrometry approaches now enables the analysis of microbial single cells — mainly by miniaturizing cell handling, incubation, and improving chip-coupling concepts for analyte ionization by interfacing microfluidic chips and mass spectrometers. This review aims at distilling the enabling principles behind microbial single-cell mass spectrometry and puts them into perspective for the future of the field.

Persistent UFZ Identifier
Schirmer, M., Dusny, C. (2023):
Microbial single-cell mass spectrometry: status, challenges, and prospects
Curr. Opin. Biotechnol. 83 , art. 102977 10.1016/j.copbio.2023.102977