Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s00374-023-01754-0 |
Licence  |
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| Title (Primary) | Amino mapping: possibility to visualize amino-N compounds in the rhizosphere of Zea Mays L. |
| Author | Khosrozadeh, S.; Guber, A.; Nourbakhsh, F.; Khalili, B.; Blagodatskaya, E. |
| Source Titel | Biology and Fertility of Soils |
| Year | 2023 |
| Department | BOOEK |
| Volume | 59 |
| Issue | 8 |
| Page From | 1007 |
| Page To | 1011 |
| Language | englisch |
| Topic | T5 Future Landscapes |
| Supplements | https://static-content.springer.com/esm/art%3A10.1007%2Fs00374-023-01754-0/MediaObjects/374_2023_1754_MOESM1_ESM.docx |
| Keywords | Time-lapse amino mapping (TLAM); OPAME reagent; Amino-N compound distribution; Rhizosphere |
| Abstract | Understanding N uptake by plants, the N cycle, and their relationship to soil heterogeneity has generated a great deal of interest in the distribution of amino-N compounds in soil. Visualization of the spatial distribution of amino-N in soil can provide insights into the role of labile N in plant-microbial mechanisms of N acquisition and plant N uptake, but until now, it has remained technically challenging. Here, we describe a novel technique to visualize the amino-N distribution at the root-soil interface. The technique is based on time-lapse amino mapping (TLAM) using membranes saturated with the fluorogenic OPAME reagent (O-phthalaldehyde and β-mercaptoethanol). OPAME in the membrane reacts with organic compounds containing a NH2 functional group at the membrane-soil interface, generating a fluorescent product visible under UV light and detectable by a digital camera. The TLAM amino-mapping technique was applied to visualize and quantify the concentration of amino-N compounds in the rhizosphere of maize (Zea Mays L.). A ten times greater amino-N concentration was detected in the rhizosphere compared to non-rhizosphere soil. The high content of amino-N was mainly associated with the root tips and was 3 times larger than the average amino-N content at seminal roots. The amino-N rhizosphere was 2 times broader around the root tips than around other parts of the roots. We concluded that TLAM is a promising approach for monitoring the fate of labile N in soils. However, the technique needs to be standardized for different soil types, plant species, and climate conditions to allow wider application. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=27233 |
| Khosrozadeh, S., Guber, A., Nourbakhsh, F., Khalili, B., Blagodatskaya, E. (2023): Amino mapping: possibility to visualize amino-N compounds in the rhizosphere of Zea Mays L. Biol. Fert. Soils 59 (8), 1007 - 1011 10.1007/s00374-023-01754-0 |
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