Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1007/s00374-023-01754-0
Licence creative commons licence
Title (Primary) Amino mapping: possibility to visualize amino-N compounds in the rhizosphere of Zea Mays L.
Author Khosrozadeh, S.; Guber, A.; Nourbakhsh, F.; Khalili, B.; Blagodatskaya, E.
Source Titel Biology and Fertility of Soils
Year 2023
Department BOOEK
Volume 59
Issue 8
Page From 1007
Page To 1011
Language englisch
Topic T5 Future Landscapes
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs00374-023-01754-0/MediaObjects/374_2023_1754_MOESM1_ESM.docx
Keywords Time-lapse amino mapping (TLAM); OPAME reagent; Amino-N compound distribution; Rhizosphere
Abstract Understanding N uptake by plants, the N cycle, and their relationship to soil heterogeneity has generated a great deal of interest in the distribution of amino-N compounds in soil. Visualization of the spatial distribution of amino-N in soil can provide insights into the role of labile N in plant-microbial mechanisms of N acquisition and plant N uptake, but until now, it has remained technically challenging. Here, we describe a novel technique to visualize the amino-N distribution at the root-soil interface. The technique is based on time-lapse amino mapping (TLAM) using membranes saturated with the fluorogenic OPAME reagent (O-phthalaldehyde and β-mercaptoethanol). OPAME in the membrane reacts with organic compounds containing a NH2 functional group at the membrane-soil interface, generating a fluorescent product visible under UV light and detectable by a digital camera. The TLAM amino-mapping technique was applied to visualize and quantify the concentration of amino-N compounds in the rhizosphere of maize (Zea Mays L.). A ten times greater amino-N concentration was detected in the rhizosphere compared to non-rhizosphere soil. The high content of amino-N was mainly associated with the root tips and was 3 times larger than the average amino-N content at seminal roots. The amino-N rhizosphere was 2 times broader around the root tips than around other parts of the roots. We concluded that TLAM is a promising approach for monitoring the fate of labile N in soils. However, the technique needs to be standardized for different soil types, plant species, and climate conditions to allow wider application.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=27233
Khosrozadeh, S., Guber, A., Nourbakhsh, F., Khalili, B., Blagodatskaya, E. (2023):
Amino mapping: possibility to visualize amino-N compounds in the rhizosphere of Zea Mays L.
Biol. Fert. Soils 59 (8), 1007 - 1011 10.1007/s00374-023-01754-0