Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1093/femsmc/xtac028
Licence creative commons licence
Title (Primary) Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
Author Abu Quba, A.A.; Goebel, M.-O.; Karagulyan, M.; Miltner, A. ORCID logo ; Kästner, M.; Bachmann, J.; Schaumann, G.E.; Diehl, D.
Source Titel FEMS Microbes
Year 2023
Department UBT
Volume 4
Page From xtac028
Language englisch
Topic T7 Bioeconomy
Supplements https://bit.ly/42EPbHQ
Keywords hypertonic osmotic stress; hydrophobicity; chemical force microscopy; cell surface; Pseudomonas fluorescens; X-ray photoelectron spectroscopy
Abstract Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influence of adaptation to hypertonic stress on cell wettability, shape, adhesion, and surface chemical composition of Pseudomonas fluorescens. From this we aim to discover possible relations between the changes in wettability of bacterial films studied by contact angle and single cells studied by atomic and chemical force microscopy (AFM, CFM), which is still lacking. We show that by stress the adhesion forces of the cell surfaces towards hydrophobic functionalized probes increase while they decrease towards hydrophilic functionalized tips. This is consistent with the contact angle results. Further, cell size shrunk and protein content increased upon stress. The results suggest two possible mechanisms: Cell shrinkage is accompanied by the release of outer membrane vesicles by which the protein to lipid ratio increases. The higher protein content increases the rigidity and the number of hydrophobic nano-domains per surface area.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=27035
Abu Quba, A.A., Goebel, M.-O., Karagulyan, M., Miltner, A., Kästner, M., Bachmann, J., Schaumann, G.E., Diehl, D. (2023):
Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
FEMS Microbes 4 , xtac028