Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.soilbio.2022.108887 |
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| Title (Primary) | Long-term N-addition alters the community structure of functionally important N-cycling soil microorganisms across global grasslands |
| Author | Frey, B.; Moser, B.; Tytgat, B.; Zimmermann, S.; Alberti, J.; Biederman, L.A.; Borer, E.T.; Broadbent, A.A.D.; Caldeira, M.C.; Davies, K.F.; Eisenhauer, N.; Eskelinen, A.; Fay, P.A.; Hagedorn, F.; Hautier, Y.; MacDougall, A.S.; McCulley, R.L.; Moore, J.L.; Nepel, M.; Power, S.A.; Seabloom, E.W.; Vázquez, E.; Virtanen, R.; Yahdjian, L.; Risch, A.C. |
| Source Titel | Soil Biology & Biochemistry |
| Year | 2023 |
| Department | PHYDIV |
| Volume | 176 |
| Page From | art. 108887 |
| Language | englisch |
| Topic | T5 Future Landscapes |
| Supplements | https://ars.els-cdn.com/content/image/1-s2.0-S0038071722003443-mmc1.pdf https://ars.els-cdn.com/content/image/1-s2.0-S0038071722003443-mmc2.docx https://ars.els-cdn.com/content/image/1-s2.0-S0038071722003443-mmc3.docx |
| Keywords | Ammonia oxidizer; Biogeography; Diazotroph; Grassland; N-cycling microbial community; N-Fertilization; N2-fixing bacteria; nifH; Nutrient network (NutNet); Urea |
| Abstract | Anthropogenic nitrogen (N) input is known to alter the soil microbiome, but how N enrichment influences the abundance, alpha-diversity and community structure of N-cycling functional microbial communities in grasslands remains poorly understood. Here, we collected soils from plant communities subjected to up to 9 years of annual N-addition (10 g N m−2 per year using urea as a N-source) and from unfertilized plots (control) in 30 grasslands worldwide spanning a large range of climatic and soil conditions. We focused on three key microbial groups responsible for two essential processes of the global N cycle: N2 fixation (soil diazotrophs) and nitrification (AOA: ammonia-oxidizing archaea and AOB: ammonia-oxidizing bacteria). We targeted soil diazotrophs, AOA and AOB using Illumina MiSeq sequencing and measured the abundance (gene copy numbers) using quantitative PCR. N-addition shifted the structure of the diazotrophic communities, although their alpha-diversity and abundance were not affected. AOA and AOB responded differently to N-addition. The abundance and alpha-diversity of AOB increased, and their community structure shifted with N-addition. In contrast, AOA were not affected by N-addition. AOA abundance outnumbered AOB in control plots under conditions of low N availability, whereas N-addition favoured copiotrophic AOB. Overall, N-addition showed a low impact on soil diazotrophs and AOA while effects for AOB communities were considerable. These results reveal that long-term N-addition has important ecological implications for key microbial groups involved in two critical soil N-cycling processes. Increased AOB abundance and community shifts following N-addition may change soil N-cycling, as larger population sizes may promote higher rates of ammonia oxidation and subsequently increase N loss via gaseous and soil N-leaching. These findings bring us a step closer to predicting the responses and feedbacks of microbial-mediated N-cycling processes to long-term anthropogenic N-addition in grasslands. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26847 |
| Frey, B., Moser, B., Tytgat, B., Zimmermann, S., Alberti, J., Biederman, L.A., Borer, E.T., Broadbent, A.A.D., Caldeira, M.C., Davies, K.F., Eisenhauer, N., Eskelinen, A., Fay, P.A., Hagedorn, F., Hautier, Y., MacDougall, A.S., McCulley, R.L., Moore, J.L., Nepel, M., Power, S.A., Seabloom, E.W., Vázquez, E., Virtanen, R., Yahdjian, L., Risch, A.C. (2023): Long-term N-addition alters the community structure of functionally important N-cycling soil microorganisms across global grasslands Soil Biol. Biochem. 176 , art. 108887 10.1016/j.soilbio.2022.108887 |
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