Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3389/fmicb.2022.1042437
Licence creative commons licence
Title (Primary) Whole-genome sequence of the filamentous diazotrophic cyanobacterium Tolypothrix sp. PCC 7712 and its comparison with non-diazotrophic Tolypothrix sp. PCC 7601
Author Bozan, M. ORCID logo ; Popp, D.; Kallies, R.; Nunes da Rocha, U.; Klähn, S.; Bühler, K.
Source Titel Frontiers in Microbiology
Year 2022
Department UMB; SOMA
Volume 13
Page From art. 1042437
Language englisch
Topic T7 Bioeconomy
Supplements https://www.frontiersin.org/articles/10.3389/fmicb.2022.1042437/full#supplementary-material
Keywords next-generation sequencing; comparative genomics; cyanobacteria; Tolypothrix; Fremyella diplosiphon
Abstract Cyanobacteria are highly promising microorganisms in forthcoming biotechnologies. Besides the systematic development of molecular tools for genetic engineering, the design of chassis strains and novel reactor concepts are in focus. The latter includes capillary biofilm reactors (CBR), which offer a high surface area-to-volume ratio and very high cell densities. In this context, Tolypothrix sp. PCC 7712 was found to be highly suited for this reactor system due to maximal surface coverage, extraordinarily strong biofilm attachment, and high biomass formation. Here, we provide the genome sequence of Tolypothrix sp. PCC 7712 to potentially allow targeted strain engineering. Surprisingly, it was almost identical to an available incomplete genome draft of Tolypothrix sp. PCC 7601. Thus, we completely sequenced this strain as well and compared it in detail to strain PCC 7712. Comparative genome analysis revealed 257 and 80 unique protein-coding sequences for strains PCC 7601 and PCC 7712, respectively. Clustering genomes based on average nucleotide identity (ANI) and 16S rRNA homology showed 99.98% similarity and only minor distance, respectively, between the two strains in contrast to 21 other cyanobacterial genomes. Despite these high similarities, both strains differ in the ability to fix atmospheric nitrogen and show specific sequence variations, which are discussed in the paper.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26795
Bozan, M., Popp, D., Kallies, R., Nunes da Rocha, U., Klähn, S., Bühler, K. (2022):
Whole-genome sequence of the filamentous diazotrophic cyanobacterium Tolypothrix sp. PCC 7712 and its comparison with non-diazotrophic Tolypothrix sp. PCC 7601
Front. Microbiol. 13 , art. 1042437