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Title (Primary) IncP-type plasmids carrying genes for antibiotic resistance or for aromatic compound degradation are prevalent in sequenced Aromatoleum and Thauera strains
Author Lo, H.-Y.; Martínez-Lavanchy, P.; Goris, T.; Heider, J.; Boll, M.; Kaster, A.-K.; Müller, J.A.
Journal Environmental Microbiology
Year 2022
Department UMB; UBT
Volume 24
Issue 12
Page From 6411
Page To 6425
Language englisch
Topic T7 Bioeconomy
Supplements https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.16262&file=emi16262-sup-0001-Supinfo.docx
https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.16262&file=emi16262-sup-0002-TableS4.xlsx
Abstract Self-transferable plasmids of the incompatibility group P-1 (IncP-1) are considered important carriers of genes for antibiotic resistance and other adaptive functions. In the laboratory, these plasmids have a broad host range; however, little is known about their in situ host profile. In this study, we discovered that Thauera aromatica K172T, a facultative denitrifying microorganism capable of degrading various aromatic compounds, contains a plasmid highly similar to the IncP-1 ε archetype pKJK5. The plasmid harbours multiple antibiotic resistance genes and is maintained in strain K172T for at least 1000 generations without selection pressure from antibiotics. In a subsequent search, we found additional nine IncP-type plasmids in a total of 40 sequenced genomes of the closely related genera Aromatoleum and Thauera. Six of these plasmids form a novel IncP-1 subgroup designated θ, four of which carry genes for anaerobic or aerobic degradation of aromatic compounds. Pentanucleotide sequence analyses (k-mer profiling) indicated that Aromatoleum spp. and Thauera spp. are among the most suitable hosts for the θ plasmids. Our results highlight the importance of IncP-1 plasmids for the genetic adaptation of these common facultative denitrifying bacteria, and provide novel insights into the in situ host profile of these plasmids.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26772
Lo, H.-Y., Martínez-Lavanchy, P., Goris, T., Heider, J., Boll, M., Kaster, A.-K., Müller, J.A. (2022):
IncP-type plasmids carrying genes for antibiotic resistance or for aromatic compound degradation are prevalent in sequenced Aromatoleum and Thauera strains
Environ. Microbiol. 24 (12), 6411 - 6425