Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1111/1462-2920.16262 |
Licence  |
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| Title (Primary) | IncP-type plasmids carrying genes for antibiotic resistance or for aromatic compound degradation are prevalent in sequenced Aromatoleum and Thauera strains |
| Author | Lo, H.-Y.; Martínez-Lavanchy, P.; Goris, T.; Heider, J.; Boll, M.; Kaster, A.-K.; Müller, J.A. |
| Source Titel | Environmental Microbiology |
| Year | 2022 |
| Department | UMB; UBT |
| Volume | 24 |
| Issue | 12 |
| Page From | 6411 |
| Page To | 6425 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.16262&file=emi16262-sup-0001-Supinfo.docx https://sfamjournals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.16262&file=emi16262-sup-0002-TableS4.xlsx |
| Abstract | Self-transferable plasmids of the incompatibility group P-1 (IncP-1) are considered important carriers of genes for antibiotic resistance and other adaptive functions. In the laboratory, these plasmids have a broad host range; however, little is known about their in situ host profile. In this study, we discovered that Thauera aromatica K172T, a facultative denitrifying microorganism capable of degrading various aromatic compounds, contains a plasmid highly similar to the IncP-1 ε archetype pKJK5. The plasmid harbours multiple antibiotic resistance genes and is maintained in strain K172T for at least 1000 generations without selection pressure from antibiotics. In a subsequent search, we found additional nine IncP-type plasmids in a total of 40 sequenced genomes of the closely related genera Aromatoleum and Thauera. Six of these plasmids form a novel IncP-1 subgroup designated θ, four of which carry genes for anaerobic or aerobic degradation of aromatic compounds. Pentanucleotide sequence analyses (k-mer profiling) indicated that Aromatoleum spp. and Thauera spp. are among the most suitable hosts for the θ plasmids. Our results highlight the importance of IncP-1 plasmids for the genetic adaptation of these common facultative denitrifying bacteria, and provide novel insights into the in situ host profile of these plasmids. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26772 |
| Lo, H.-Y., Martínez-Lavanchy, P., Goris, T., Heider, J., Boll, M., Kaster, A.-K., Müller, J.A. (2022): IncP-type plasmids carrying genes for antibiotic resistance or for aromatic compound degradation are prevalent in sequenced Aromatoleum and Thauera strains Environ. Microbiol. 24 (12), 6411 - 6425 10.1111/1462-2920.16262 |
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