Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/bioengineering9110678
Licence creative commons licence
Title (Primary) Effect of inoculum microbial diversity in ex situ biomethanation of hydrogen
Author Logroño, W.; Kluge, P.; Kleinsteuber, S. ORCID logo ; Harms, H.; Nikolausz, M.
Source Titel Bioengineering-Basel
Year 2022
Department UMB
Volume 9
Issue 11
Page From art. 678
Language englisch
Topic T7 Bioeconomy
Keywords power-to-gas; renewable energy; biomethane; hydrogen; hydrogenotrophic methanogens; homoacetogenesis; methanogenic communities
Abstract The effects of the inoculum origin, temperature or operational changes on ex situ biomethanation by complex microbial communities have been investigated; however, it remains unclear how the diversity of the inoculum influences the process and its stability. We explored the effect of microbial diversity of four inocula (coded as PF, WW, S37 and Nrich) on methane production, process stability and the formation of volatile fatty acids as by-products. The highest methane amounts produced were 3.38 ± 0.37 mmol, 3.20 ± 0.07 mmol, 3.07 ± 0.27 mmol and 3.14 ± 0.06 mmol for PF, WW, S37 and Nrich, respectively. The highest acetate concentration was found in less diverse cultures (1679 mg L−1 and 1397 mg L−1 for S37 and Nrich, respectively), whereas the acetate concentrations remained below 30 mg L−1 in the more diverse cultures. The maximum concentration of propionate was observed in less diverse cultures (240 mg L−1 and 37 mg L−1 for S37 and Nrich cultures, respectively). The highly diverse cultures outperformed the medium and low diversity cultures in the long-term operation. Methanogenic communities were mainly composed of hydrogenotrophic methanogens in all cultures. Aceticlastic methanogenesis was only active in the highly diverse sludge community throughout the experiment. The more diverse the inocula, the more methane was produced and the less volatile fatty acids accumulated, which could be attributed to the high number of microbial functions working together to keep a stable and balanced process. It is concluded that the inoculum origin and its diversity are very important factors to consider when the biomethanation process is performed with complex microbial communities.
Persistent UFZ Identifier
Logroño, W., Kluge, P., Kleinsteuber, S., Harms, H., Nikolausz, M. (2022):
Effect of inoculum microbial diversity in ex situ biomethanation of hydrogen
Bioengineering-Basel 9 (11), art. 678 10.3390/bioengineering9110678