Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/molecules27217568
Licence creative commons licence
Title (Primary) Evaluation of antioxidant activity and biotransformation of Opuntia ficus fruit: The effect of in vitro and ex vivo gut microbiota metabolism
Author Sallam, I.E.; Rolle-Kampczyk, U.; Schäpe, S.S.; Zaghloul, S.S.; El Dine, R.S.; Shao, P.; von Bergen, M.; Farag, M.A.
Source Titel Molecules
Year 2022
Department iDiv; MOLSYB
Volume 27
Issue 21
Page From art. 7568
Language englisch
Topic T9 Healthy Planet
Supplements https://www.mdpi.com/article/10.3390/molecules27217568/s1
Keywords O. ficus-indica; gut microbiota; biotransformation; UHPLC-QTOF-MS; chemometrics; antioxidant; DPPH; ORAC; FRAP
Abstract Opuntia ficus-indica biological effects are attributed to several bioactive metabolites. However, these actions could be altered in vivo by biotransformation reactions mainly via gut microbiota. This study assessed gut microbiota effect on the biotransformation of O. ficus-indica metabolites both in vitro and ex vivo. Two-time aliquots (0.5 and 24 h) from the in vitro assay were harvested post incubation of O. ficus-indica methanol extract with microbial consortium, while untreated and treated samples with fecal bacterial culture from the ex vivo assay were prepared. Metabolites were analyzed using UHPLC-QTOF-MS, with flavonoid glycosides completely hydrolyzed in vitro at 24 h being converted to two major metabolites, 3-(4-hydroxyphenyl)propanoic acid and phloroglucinol, concurrent with an increase in the gallic acid level. In case of the ex vivo assay, detected flavonoid glycosides in untreated sample were completely absent from treated counterpart with few flavonoid aglycones and 3-(4-hydroxyphenyl)propanoic acid in parallel to an increase in piscidic acid. In both assays, fatty and organic acids were completely hydrolyzed being used as energy units for bacterial growth. Chemometric tools were employed revealing malic and (iso)citric acids as the main discriminating metabolites in vitro showing an increased abundance at 0.5 h, whereas in ex vivo assay, (iso)citric, aconitic and mesaconic acids showed an increase at untreated sample. Piscidic acid was a significant marker for the ex vivo treated sample. DPPH, ORAC and FRAP assays were further employed to determine whether these changes could be associated with changes in antioxidant activity, and all assays showed a decline in antioxidant potential post biotransformation.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26742
Sallam, I.E., Rolle-Kampczyk, U., Schäpe, S.S., Zaghloul, S.S., El Dine, R.S., Shao, P., von Bergen, M., Farag, M.A. (2022):
Evaluation of antioxidant activity and biotransformation of Opuntia ficus fruit: The effect of in vitro and ex vivo gut microbiota metabolism
Molecules 27 (21), art. 7568 10.3390/molecules27217568