Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1111/1462-2920.16302
Licence creative commons licence
Title (Primary) Metabolic reconstruction of the near complete microbiome of the model sponge Ianthella basta
Author Engelberts, J.P.; Robbins, S.J.; Herbold, C.W.; Moeller, F.U.; Jehmlich, N. ORCID logo ; Laffy, P.W.; Wagner, M.; Webster, N.S.
Source Titel Environmental Microbiology
Year 2023
Department MOLSYB
Volume 25
Issue 3
Page From 646
Page To 660
Language englisch
Topic T9 Healthy Planet
Abstract Many marine sponges host highly diverse microbiomes that contribute to various aspects of host health. Although the putative function of individual groups of sponge symbionts has been increasingly described, the extreme diversity has generally precluded in-depth characterisation of entire microbiomes, including identification of syntrophic partnerships. The Indo-Pacific sponge Ianthella basta is emerging as a model organism for symbiosis research, hosting only three dominant symbionts: a Thaumarchaeotum, a Gammaproteobacterium, and an Alphaproteobacterium and a range of other low abundance or transitory taxa. Here, we retrieved metagenome assembled genomes (MAGs) representing >90% of I. basta's microbial community, facilitating the metabolic reconstruction of the sponge's near complete microbiome. Through this analysis, we identified metabolic complementarity between microbes, including vitamin sharing, described the importance of low abundance symbionts, and characterised a novel microbe-host attachment mechanism in the Alphaproteobacterium. We further identified putative viral sequences, highlighting the role viruses can play in maintaining symbioses in I. basta through the horizontal transfer of eukaryotic-like proteins, and complemented this data with metaproteomics to identify active metabolic pathways in bacteria, archaea, and viruses. This data provides the framework to adopt I. basta as a model organism for studying host-microbe interactions and provides a basis for in-depth physiological experiments.
Persistent UFZ Identifier
Engelberts, J.P., Robbins, S.J., Herbold, C.W., Moeller, F.U., Jehmlich, N., Laffy, P.W., Wagner, M., Webster, N.S. (2023):
Metabolic reconstruction of the near complete microbiome of the model sponge Ianthella basta
Environ. Microbiol. 25 (3), 646 - 660 10.1111/1462-2920.16302