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Title (Primary) Water stress-driven changes in bacterial cell surface properties
Author Karagulyan, M.; Goebel, M.-O.; Diehl, D.; Abu Quba, A.A.; Kästner, M.; Bachmann, J.; Wick, L.Y.; Schaumann, G.E.; Miltner, A.
Journal Applied and Environmental Microbiology
Year 2022
Department UMB; UBT
Volume 88
Issue 21
Page From e00732-22
Language englisch
Topic T7 Bioeconomy
Supplements https://journals.asm.org/doi/suppl/10.1128/aem.00732-22/suppl_file/aem.00732-22-s0001.pdf
https://journals.asm.org/doi/suppl/10.1128/aem.00732-22/suppl_file/aem.00732-22-s0002.xlsx
Keywords stress response; soil bacteria; cell surface hydrophobicity; cell surfacephysicochemical properties
Abstract Increased drought intensity and frequency exposes soil bacteria to prolonged water stress. While numerous studies reported on behavioral and physiological mechanisms of bacterial adaptation to water stress, changes in bacterial cell surface properties during adaptation are not well researched. We studied adaptive changes in cell surface hydrophobicity (CSH) after exposure to osmotic (NaCl) and matric stress (polyethylene glycol 8000 [PEG 8000]) for six typical soil bacteria (Bacillus subtilis, Arthrobacter chlorophenolicus, Pseudomonas fluorescens, Novosphingobium aromaticivorans, Rhodococcus erythropolis, and Mycobacterium pallens) covering a wide range of cell surface properties. Additional physicochemical parameters (surface chemical composition, surface charge, cell size and stiffness) of B. subtilis and P. fluorescens were analyzed to understand their possible contribution to CSH development. Changes in CSH caused by osmotic and matric stress depend on strain and stress type. CSH of B. subtilis and P. fluorescens increased with stress intensity, R. erythropolis and M. pallens exhibited a generally high but constant contact angle, while the response of A. chlorophenolicus and N. aromaticivorans depended on growth conditions and stress type. Osmotically driven changes in CSH of B. subtilis and P. fluorescens are accompanied by increasing surface N/C ratio, suggesting an increase in protein concentration within the cell wall. Cell envelope proteins thus presumably control bacterial CSH in two ways: (i) by increases in the relative density of surface proteins due to efflux of cytoplasmic water and subsequent cell shrinkage, and (ii) by destabilization of cell wall proteins, resulting in conformational changes which render the surface more hydrophobic.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26659
Karagulyan, M., Goebel, M.-O., Diehl, D., Abu Quba, A.A., Kästner, M., Bachmann, J., Wick, L.Y., Schaumann, G.E., Miltner, A. (2022):
Water stress-driven changes in bacterial cell surface properties
Appl. Environ. Microb. 88 (21), e00732-22