Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1002/tox.20162
Title (Primary) Cytotoxicity assessment of gliotoxin and penicillic acid in Tetrahymena pyriformis
Author Gräbsch, C.; Wichmann, G.; Loffhagen, N.; Herbarth, O.; Müller, A.
Source Titel Environmental Toxicology
Year 2006
Volume 21
Issue 2
Page From 111
Page To 117
Language englisch
Keywords mycotoxins; gliotoxin; penicillic acid; Tetrahymena pyriformis; cellular ATP content; oxygen consumption; MTT assay
Abstract Various studies have documented the associations between mold exposure and effects on health. Mycotoxins, which occur in spores and mold fragments, can be involved in processes that have pathological effects, such as adynamia of the immune system, recurrent infections of the respiratory tract, or asthma. Using Tetrahymena pyriformis, a single-cell organism well established as a suitable model for human respiratory epithelium-cell functionalities, we investigated dose-response relationships of the mycotoxins gliotoxin and penicillic acid. Our study focused on the viability (cell count, MTT assay), energy levels (adenosine-5-triphosphate content), energy-providing processes (MTT reduction per cell), and cell respiration (oxygen consumption). Both mycotoxins acted as cytotoxins in a dose-dependent manner. Gliotoxin had a stronger inhibitory effect (EC50 0.38 M) than did penicillic acid (EC50 343.19 M). The energy-providing processes were not inhibited or were only weakly inhibited under the influence of gliotoxin, whereas penicillic acid caused stimulation of the physiological parameters. Summarizing the results, it is clear that the two investigated mycotoxins must have different modes of action. They are not only different in the strength of their toxic effects but also in a variety of physiological aspects. In addition, T. pyriformis showed differences in its ability to overcome the negative effects of particular mycotoxin exposures. © 2006 Wiley Periodicals, Inc. Environ Toxicol 21: 111-117, 2006.
Persistent UFZ Identifier
Gräbsch, C., Wichmann, G., Loffhagen, N., Herbarth, O., Müller, A. (2006):
Cytotoxicity assessment of gliotoxin and penicillic acid in Tetrahymena pyriformis
Environ.Toxicol. 21 (2), 111 - 117 10.1002/tox.20162