Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.3390/cells11172690 |
Licence  |
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| Title (Primary) | IL-10 producing B cells protect against LPS-induced murine preterm birth by promoting PD1- and ICOS-expressing T cells |
| Author | Busse, M.; Zenclussen, A.C. |
| Source Titel | Cells |
| Year | 2022 |
| Department | IMMU |
| Volume | 11 |
| Issue | 17 |
| Page From | art. 2690 |
| Language | englisch |
| Topic | T9 Healthy Planet |
| Supplements | Supplement 1 |
| Keywords | B cells; IL-10; B10 cells; regulatory B cells; programmed cell death protein-1 (PD-); inducible costimulator (ICOS); preterm birth |
| Abstract | B cells and in particular IL-10-secreting B cells emerge as important players in immune balance during pregnancy. We have recently revealed that CD19-deficient (CD19−/−), B cell-specific IL-10-deficient (BIL-10−/−) and B cell-deficient µMT pregnant mice are highly susceptible to LPS-induced preterm birth (PTB). We aimed to analyze the ability of IL-10-secreting cells to protect from PTB and the underlying mechanisms. Wild type (WT), CD19−/−, BIL-10−/− and µMT mice were treated with LPS at gd16 and the cellular immune response was investigated 24 h later. LPS-treated BIL-10−/− dams showed a more pronounced PTB phenotype compared to WT, CD19−/− and µMT females, and increased inflammatory and reduced anti-inflammatory mediator concentrations in the peritoneal cavity and serum. CD19−/−, BIL-10−/− and µMT mice displayed altered immune cell population frequencies in the blood and uterus with lower numbers of IL-10-secreting B cells and T cells. BIL-10−/− mothers presented decreased frequencies of uterine CD4+CD25+Foxp3+ Treg cells. Co-stimulatory molecules are critical for feto-maternal tolerance and IL-10 secretion. We found dysregulated PD-1 expression in peripheral blood and ICOS expression in the uterus of CD19−/−, BIL-10−/− and µMT dams. Our data show that B cell-specific IL-10-signaling is essential for a balanced maternal immune response to an inflammatory stimulant that cannot be hampered without IL-10-secreting B cells. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26509 |
| Busse, M., Zenclussen, A.C. (2022): IL-10 producing B cells protect against LPS-induced murine preterm birth by promoting PD1- and ICOS-expressing T cells Cells 11 (17), art. 2690 10.3390/cells11172690 |
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