Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1038/s41467-022-31712-7 |
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| Title (Primary) | Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis |
| Author | Zhang, H.; Quintana, J.; Ütkür, K.; Adrian, L.; Hawer, H.; Mayer, K.; Gong, X.; Castanedo, L.; Schulten, A.; Janina, N.; Peters, M.; Wirtz, M.; Brinkmann, U.; Schaffrath, R.; Krämer, U. |
| Source Titel | Nature Communications |
| Year | 2022 |
| Department | UBT |
| Volume | 13 |
| Page From | art. 4009 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-022-31712-7/MediaObjects/41467_2022_31712_MOESM1_ESM.pdf https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-022-31712-7/MediaObjects/41467_2022_31712_MOESM3_ESM.pdf https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-022-31712-7/MediaObjects/41467_2022_31712_MOESM4_ESM.xlsx https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-022-31712-7/MediaObjects/41467_2022_31712_MOESM5_ESM.xlsx https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-022-31712-7/MediaObjects/41467_2022_31712_MOESM6_ESM.xlsx https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-022-31712-7/MediaObjects/41467_2022_31712_MOESM8_ESM.xlsx |
| Abstract | Diphthamide, a post-translationally modified histidine residue of eukaryotic TRANSLATION ELONGATION FACTOR2 (eEF2), is the human host cell-sensitizing target of diphtheria toxin. Diphthamide biosynthesis depends on the 4Fe-4S-cluster protein Dph1 catalyzing the first committed step, as well as Dph2 to Dph7, in yeast and mammals. Here we show that diphthamide modification of eEF2 is conserved in Arabidopsis thaliana and requires AtDPH1. Ribosomal −1 frameshifting-error rates are increased in Arabidopsis dph1 mutants, similar to yeast and mice. Compared to the wild type, shorter roots and smaller rosettes of dph1 mutants result from fewer formed cells. TARGET OF RAPAMYCIN (TOR) kinase activity is attenuated, and autophagy is activated, in dph1 mutants. Under abiotic stress diphthamide-unmodified eEF2 accumulates in wild-type seedlings, most strongly upon heavy metal excess, which is conserved in human cells. In summary, our results suggest that diphthamide contributes to the functionality of the translational machinery monitored by plants to regulate growt |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26421 |
| Zhang, H., Quintana, J., Ütkür, K., Adrian, L., Hawer, H., Mayer, K., Gong, X., Castanedo, L., Schulten, A., Janina, N., Peters, M., Wirtz, M., Brinkmann, U., Schaffrath, R., Krämer, U. (2022): Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis Nat. Commun. 13 , art. 4009 10.1038/s41467-022-31712-7 |
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