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Title (Primary) Acetyl-CoA-Carboxylase 1-mediated de novo fatty acid synthesis sustains Lgr5+ intestinal stem cell function
Author Li, S.; Lu, C.-W.; Diem, E.C.; Li, W.; Guderian, M.; Lindenberg, M.; Kruse, F.; Buettner, M.; Floess, S.; Winny, M.R.; Geffers, R.; Richnow, H.-H.; Abraham, W.-R.; Grassl, G.A.; Lochner, M.
Journal Nature Communications
Year 2022
Department ISOBIO
Volume 13
Page From art. 3998
Language englisch
Topic T7 Bioeconomy
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-022-31725-2/MediaObjects/41467_2022_31725_MOESM1_ESM.pdf
Abstract Basic processes of the fatty acid metabolism have an important impact on the function of intestinal epithelial cells (IEC). However, while the role of cellular fatty acid oxidation is well appreciated, it is not clear how de novo fatty acid synthesis (FAS) influences the biology of IECs. We report here that interfering with de novo FAS by deletion of the enzyme Acetyl-CoA-Carboxylase (ACC)1 in IECs results in the loss of epithelial crypt structures and a specific decline in Lgr5+ intestinal epithelial stem cells (ISC). Mechanistically, ACC1-mediated de novo FAS supports the formation of intestinal organoids and the differentiation of complex crypt structures by sustaining the nuclear accumulation of PPAR╬┤/╬▓-catenin in ISCs. The dependency of ISCs on cellular de novo FAS is tuned by the availability of environmental lipids, as an excess delivery of external fatty acids is sufficient to rescue the defect in crypt formation. Finally, inhibition of ACC1 reduces the formation of tumors in colitis-associated colon cancer, together highlighting the importance of cellular lipogenesis for sustaining ISC function and providing a potential perspective to colon cancer therapy.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26394
Li, S., Lu, C.-W., Diem, E.C., Li, W., Guderian, M., Lindenberg, M., Kruse, F., Buettner, M., Floess, S., Winny, M.R., Geffers, R., Richnow, H.-H., Abraham, W.-R., Grassl, G.A., Lochner, M. (2022):
Acetyl-CoA-Carboxylase 1-mediated de novo fatty acid synthesis sustains Lgr5+ intestinal stem cell function
Nat. Commun. 13 , art. 3998