Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1098/rspb.2022.0130
Title (Primary) Cross-kingdom interactions and functional patterns of active microbiota matter in governing deadwood decay
Author Purahong, W.; Tanunchai, B.; Muszynski, S.; Maurer, F.; Wahdan, S.F.M.; Malter, J.; Buscot, F.; Noll, M.
Source Titel Proceedings of the Royal Society B-Biological Sciences
Year 2022
Department BOOEK; iDiv
Volume 289
Issue 1974
Page From art. 20220130
Language englisch
Topic T5 Future Landscapes
Data and Software links
Keywords enzyme activity; microbiome; spruce; beech; diazotrophs deadwood decay; bromodeoxyuridine
Abstract Microbial community members are the primary microbial colonizers and active decomposers of deadwood. This study placed sterilized standardized beech and spruce sapwood specimens on the forest ground of 8 beech- and 8 spruce-dominated forest sites. After 370 days, specimens were assessed for mass loss, nitrogen (N) content and 15N isotopic signature, hydrolytic and lignin-modifying enzyme activities. Each specimen was incubated with bromodeoxyuridine (BrdU) to label metabolically active fungal and bacterial community members, which were assessed using amplicon sequencing. Fungal saprotrophs colonized the deadwood accompanied by a distinct bacterial community that was capable of cellulose degradation, aromatic depolymerization, and N2 fixation. The latter were governed by the genus Sphingomonas, which was co-present with the majority of saprotrophic fungi regardless of whether beech or spruce specimens were decayed. Moreover, the richness of the diazotrophic Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium group was significantly correlated with mass loss, N content and 15N isotopic signature. By contrast, presence of obligate predator Bdellovibrio spp. shifted bacterial community composition and were linked to decreased beech deadwood decay rates. Our study provides the first account of the composition and function of metabolically active wood-colonizing bacterial and fungal communities, highlighting cross-kingdom interactions during the early and intermediate stages of wood decay.
Persistent UFZ Identifier
Purahong, W., Tanunchai, B., Muszynski, S., Maurer, F., Wahdan, S.F.M., Malter, J., Buscot, F., Noll, M. (2022):
Cross-kingdom interactions and functional patterns of active microbiota matter in governing deadwood decay
Proc. R. Soc. B-Biol. Sci. 289 (1974), art. 20220130 10.1098/rspb.2022.0130