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Title (Primary) The potential of multispectral imaging flow cytometry for environmental monitoring
Author Dunker, S.; Boyd, M.; Durka, W.; Erler, S.; Harpole, W.S.; Henning, S.; Herzschuh, U.; Hornick, T.; Knight, T.; Lips, S.; Mäder, P.; Motivans Švara, E.; Mozarowski, S.; Rakosy, D.; Römermann, C.; Schmitt-Jansen, M.; Stoof-Leichsenring, K.; Stratmann, F.; Treudler, R.; Virtanen, R.; Wendt-Potthoff, K.; Wilhelm, C.
Journal Cytometry Part A
Year 2022
Department BIOTOX; BZF; SEEFO; iDiv; PHYDIV
Volume 101
Issue 9
Page From 782
Page To 799
Language englisch
Topic T5 Future Landscapes
Data links https://doi.org/10.48758/ufz.12577
Keywords environmental monitoring; imaging flow cytometry; plant traits
Abstract Environmental monitoring involves the quantification of microscopic cells and parti-cles such as algae, plant cells, pollen, or fungal spores. Traditional methods using con-ventional microscopy require expert knowledge, are time-intensive and not well-suited for automated high throughput. Multispectral imaging flow cytometry (MIFC)allows measurement of up to 5000 particles per second from a fluid suspension andcan simultaneously capture up to 12 images of every single particle for brightfieldand different spectral ranges, with up to 60x magnification. The high throughput ofMIFC has high potential for increasing the amount and accuracy of environmentalmonitoring, such as for plant-pollinator interactions, fossil samples, air, water or foodquality that currently rely on manual microscopic methods. Automated recognition ofparticles and cells is also possible, when MIFC is combined with deep-learning com-putational techniques. Furthermore, various fluorescence dyes can be used to stainspecific parts of the cell to highlight physiological and chemical features including:vitality of pollen or algae, allergen content of individual pollen, surface chemical com-position (carbohydrate coating) of cells, DNA- or enzyme-activity staining. Here, weoutline the great potential for MIFC in environmental research for a variety ofresearch fields and focal organisms. In addition, we provide best practicerecommendations.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26168
Dunker, S., Boyd, M., Durka, W., Erler, S., Harpole, W.S., Henning, S., Herzschuh, U., Hornick, T., Knight, T., Lips, S., Mäder, P., Motivans Švara, E., Mozarowski, S., Rakosy, D., Römermann, C., Schmitt-Jansen, M., Stoof-Leichsenring, K., Stratmann, F., Treudler, R., Virtanen, R., Wendt-Potthoff, K., Wilhelm, C. (2022):
The potential of multispectral imaging flow cytometry for environmental monitoring
Cytom. Part A 101 (9), 782 - 799