Publication Details

Category Text Publication
Reference Category Book chapters
DOI 10.1016/bs.mie.2022.03.018
Title (Primary) Analysing intracellular isoprenoid metabolites in diverse prokaryotic and eukaryotic microbes
Title (Secondary) Carotenoids: Carotenoid and apocarotenoid analysis
Author Plan, M.; Bongers, M.; Bydder, S.; Fabris, M.; Hodson, M.P.; Kelly, E.; Krömer, J.; Perez-Gil, J.; Peng, B.; Satta, A.; Schrübbers, L.C.; Vickers, C.E.
Publisher Wurtzel, E.T.
Journal Methods in Enzymology
Year 2022
Department SOMA
Volume 670
Page From 235
Page To 284
Language englisch
Topic T7 Bioeconomy
Keywords Isoprenoids; Terpenoids; Intracellular metabolites; Mevalonate pathway; Methylerythritol phosphate pathway; Prenyl phosphate metabolism; Saccharomyces cerevisiae Escherichia coli Phaeodactylum tricornutum Chlamydomonas reinhardtii Synechocystis sp. PCC 6803
Abstract Isoprenoids, also known as terpenes or terpenoids, are a very large and diverse group of natural compounds. These compounds fulfil a myriad of critical roles in biology as well as having a wide range of industrial uses. Isoprenoids are produced via two chemically distinct metabolic pathways, the mevalonate (MVA) pathway and the methylerythritol phosphate (MEP) pathway. Downstream of these two pathways is the shared prenyl phosphate pathway. Because of their importance in both basic physiology and industrial biotechnology, extraction, identification, and quantification of isoprenoid pathway intermediates is an important protocol. Here we describe methods for extraction and analysis of intracellular metabolites from the MVA, MEP, and prenyl phosphate pathways for five key model microbes: the yeast Saccharomyces cerevisiae, the bacterium Escherichia coli, the diatom Phaeodactylum tricornutum, the green algae Chlamydomonas reinhardtii, and the cyanobacterium Synechocystis sp. PCC 6803. These methods also detect several central carbon intermediates. These protocols will likely work effectively, or be readily adaptable, to a variety of related microorganisms and metabolic pathways.
Persistent UFZ Identifier
Plan, M., Bongers, M., Bydder, S., Fabris, M., Hodson, M.P., Kelly, E., Krömer, J., Perez-Gil, J., Peng, B., Satta, A., Schrübbers, L.C., Vickers, C.E. (2022):
Analysing intracellular isoprenoid metabolites in diverse prokaryotic and eukaryotic microbes
In: Wurtzel, E.T. (ed.)
Carotenoids: Carotenoid and apocarotenoid analysis
Methods Enzymol. 670
Elsevier, p. 235 - 284