Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.bbamem.2022.183953
Document author version
Title (Primary) Impact of cholesterol and sphingomyelin on intrinsic membrane permeability
Author Dahley, C.; Garessus, E.D.G.; Ebert, A. ORCID logo ; Goss, K.-U.
Source Titel Biochimica et Biophysica Acta-Biomembranes
Year 2022
Department AUC
Volume 1864
Issue 9
Page From art. 183953
Language englisch
Topic T9 Healthy Planet
Keywords Passive permeability; Black lipid membrane; Caco-2; MDCK; Cholesterol; Sphingomyelin
Abstract Transwell experiments with Caco-2 or MDCK cells are the gold standard for determining the intestinal permeability of chemicals. The intrinsic membrane permeability (P0), that can be extracted from these experiments, might be comparable to P0 measured in black lipid membrane (BLM) experiments and P0 predicted by the solubility-diffusion model. Unfortunately, the overlap between experimental P0,Caco-2/MDCK and P0,BLM data is very small. So far, differences between both approaches have been attributed to the cholesterol and sphingomyelin content of cell membranes, but the database is too sparse to thoroughly test this theory. To create a diverse dataset, we measured P0,BLM of ten chemicals in BLM experiments using DPhPC and DPhPC/cholesterol/sphingomyelin membranes. The results were compared to predicted BLM data and experimental Caco-2/MDCK data obtained from literature. While P0,BLM of all chemicals was well predicted by the solubility-diffusion model, P0,Caco-2/MDCK was only predictable for rather hydrophilic compounds with logarithmic hexadecane/water partition coefficients below −0.5. The effect of cholesterol and sphingomyelin on P0,BLM was negligibly small.
Persistent UFZ Identifier
Dahley, C., Garessus, E.D.G., Ebert, A., Goss, K.-U. (2022):
Impact of cholesterol and sphingomyelin on intrinsic membrane permeability
Biochim. Biophys. Acta-Biomembr. 1864 (9), art. 183953 10.1016/j.bbamem.2022.183953