Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1111/nph.18139 |
Licence  |
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| Title (Primary) | The transcriptional regulator RbcR controls ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes in the cyanobacterium Synechocystis sp. PCC 6803 |
| Author | Bolay, P.; Schlüter, S.; Grimm, S.; Riediger, M.; Hess, W.R.; Klähn, S. |
| Source Titel | New Phytologist |
| Year | 2022 |
| Department | SOMA |
| Volume | 235 |
| Issue | 2 |
| Page From | 432 |
| Page To | 445 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | https://nph.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2Fnph.18139&file=nph18139-sup-0001-SupInfo.pdf https://nph.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2Fnph.18139&file=nph18139-sup-0002-TableS3-S5.xlsx |
| Keywords | Calvin-Benson-Bassham cycle; Cyanobacteria; Gene regulation; RuBisCO; Synechocystis |
| Abstract | Oxygenic
photosynthesis evolved in cyanobacteria, primary producers of striking
ecological importance. Like plants, cyanobacteria use the Calvin-Benson-Bassham
cycle for CO2 fixation, fueled by ribulose-1,5-bisphosphate
carboxylase/oxygenase (RuBisCO). In a competitive reaction this enzyme is also
fixing O2 which makes it rather ineffective. To mitigate this problem,
cyanobacteria evolved a CO2 concentrating mechanism (CCM) to pool CO2 in the
vicinity of RuBisCO. However, the regulation of these carbon assimilatory
systems is only partially understood. Using the model Synechocystis sp. PCC 6803 we characterized an essential LysR-type transcriptional regulator encoded by gene sll0998. Transcript profiling of a knockdown mutant revealed diminished expression of several genes involved in carbon acquisition, including rbcLXS, sbtA and ccmKL encoding RuBisCO and parts of the CCM, respectively. We demonstrate that the Sll0998 protein binds the rbcL promoter and acts as a RuBisCO regulator (RbcR). We propose ATTA(G/A)-N5-(C/T)TAAT as the binding motif consensus. Our data validate RbcR as a regulator of inorganic carbon assimilation and define the regulon controlled by it. Biological CO2 fixation can sustain efforts to reduce its atmospheric concentrations and is fundamental for the light-driven production of chemicals directly from CO2. Information about the involved regulatory and physiological processes is crucial to engineer cyanobacterial cell factories |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25976 |
| Bolay, P., Schlüter, S., Grimm, S., Riediger, M., Hess, W.R., Klähn, S. (2022): The transcriptional regulator RbcR controls ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes in the cyanobacterium Synechocystis sp. PCC 6803 New Phytol. 235 (2), 432 - 445 10.1111/nph.18139 |
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