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Title (Primary) The transcriptional regulator RbcR controls ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes in the cyanobacterium Synechocystis sp. PCC 6803
Author Bolay, P.; Schlüter, S.; Grimm, S.; Riediger, M.; Hess, W.R.; Klähn, S.
Journal New Phytologist
Year 2022
Department SOMA
Volume 235
Issue 2
Page From 432
Page To 445
Language englisch
Topic T7 Bioeconomy
Supplements https://nph.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2Fnph.18139&file=nph18139-sup-0001-SupInfo.pdf
https://nph.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2Fnph.18139&file=nph18139-sup-0002-TableS3-S5.xlsx
Keywords Calvin-Benson-Bassham cycle; Cyanobacteria; Gene regulation; RuBisCO; Synechocystis
Abstract Oxygenic photosynthesis evolved in cyanobacteria, primary producers of striking ecological importance. Like plants, cyanobacteria use the Calvin-Benson-Bassham cycle for CO2 fixation, fueled by ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). In a competitive reaction this enzyme is also fixing O2 which makes it rather ineffective. To mitigate this problem, cyanobacteria evolved a CO2 concentrating mechanism (CCM) to pool CO2 in the vicinity of RuBisCO. However, the regulation of these carbon assimilatory systems is only partially understood.
Using the model Synechocystis sp. PCC 6803 we characterized an essential LysR-type transcriptional regulator encoded by gene sll0998. Transcript profiling of a knockdown mutant revealed diminished expression of several genes involved in carbon acquisition, including rbcLXS, sbtA and ccmKL encoding RuBisCO and parts of the CCM, respectively.
We demonstrate that the Sll0998 protein binds the rbcL promoter and acts as a RuBisCO regulator (RbcR). We propose ATTA(G/A)-N5-(C/T)TAAT as the binding motif consensus. Our data validate RbcR as a regulator of inorganic carbon assimilation and define the regulon controlled by it.
Biological CO2 fixation can sustain efforts to reduce its atmospheric concentrations and is fundamental for the light-driven production of chemicals directly from CO2. Information about the involved regulatory and physiological processes is crucial to engineer cyanobacterial cell factories
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25976
Bolay, P., Schlüter, S., Grimm, S., Riediger, M., Hess, W.R., Klähn, S. (2022):
The transcriptional regulator RbcR controls ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes in the cyanobacterium Synechocystis sp. PCC 6803
New Phytol. 235 (2), 432 - 445