Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3390/fermentation9010060
Licence creative commons licence
Title (Primary) Effect of alkaline and mechanical pretreatment of wheat straw on methanogenic enrichment cultures from Pachnoda marginata larva gut
Author Grosch Schroeder, B.; İstanbullu, H.B.; Schmidt, M.; Logroño, W.; Harms, H.; Nikolausz, M.
Journal Fermentation
Year 2023
Department ISOBIO; UMB
Volume 9
Issue 1
Page From art. 60
Language englisch
Topic T7 Bioeconomy
Supplements https://www.mdpi.com/article/10.3390/fermentation9010060/s1
Keywords alkaline pretreatment; wheat straw; lignocellulosic biomass; anaerobic digestion; larvae gut enrichment; methane; carboxylates
UFZ wide themes ProVIS;
Abstract In order to partially mimic the efficient lignocellulose pretreatment process performed naturally in the gut system of Pachnoda marginata larvae, two wheat straw pretreatments were evaluated: a mechanical pretreatment via cutting the straw into two different sizes and an alkaline pretreatment with calcium hydroxide. After pretreatment, gut enrichment cultures on wheat straw at alkaline pH were inoculated and kept at mesophilic conditions over 45 days. The methanogenic community was composed mainly of the Methanomicrobiaceae and Methanosarcinaceae families. The combined pretreatment, size reduction and alkaline pretreatment, was the best condition for methane production. The positive effect of the straw pretreatment was higher in the midgut cultures, increasing the methane production by 192%, while for hindgut cultures the methane production increased only by 149% when compared to non-pretreated straw. Scanning electron microscopy (SEM) showed that the alkaline pretreatment modified the surface of the wheat straw fibers, which promoted biofilm formation and microbial growth. The enrichment cultures derived from larva gut microbiome were able to degrade larger 1 mm alkaline treated and smaller 250 µm but non-pretreated straw at the same efficiency. The combination of mechanical and alkaline pretreatments resulted in increased, yet not superimposed, methane yield.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25967
Grosch Schroeder, B., İstanbullu, H.B., Schmidt, M., Logroño, W., Harms, H., Nikolausz, M. (2023):
Effect of alkaline and mechanical pretreatment of wheat straw on methanogenic enrichment cultures from Pachnoda marginata larva gut
Fermentation 9 (1), art. 60