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Title (Primary) Enrichment of anaerobic microbial communities from midgut and hindgut of sun beetle larvae (Pachnoda marginata) on wheat straw: effect of inoculum preparation
Author Grosch Schroeder, B.; Logroño, W.; Nunes da Rocha, U.; Harms, H.; Nikolausz, M.
Journal Microorganisms
Year 2022
Department UMB
Volume 10
Issue 4
Page From art. 761
Language englisch
Topic T7 Bioeconomy
Supplements https://www.mdpi.com/2076-2607/10/4/761/s1
Keywords enrichment; larva gut; lignocellulosic biomass; anaerobic digestion; methane; carboxylates
Abstract The Pachnoda marginata larva have complex gut microbiota capable of the effective conversion of lignocellulosic biomass. Biotechnological utilization of these microorganisms in an engineered system can be achieved by establishing enrichment cultures using a lignocellulosic substrate. We established enrichment cultures from contents of the midgut and hindgut of the beetle larva using wheat straw in an alkaline medium at mesophilic conditions. Two different inoculation preparations were used: procedure 1 (P1) was performed in a sterile bench under oxic conditions using 0.4% inoculum and small gauge needles. Procedure 2 (P2) was carried out under anoxic conditions using more inoculum (4%) and bigger gauge needles. Higher methane production was achieved with P2, while the highest acetic acid concentrations were observed with P1. In the enrichment cultures, the most abundant bacterial families were Dysgonomonadaceae, Heliobacteriaceae, Ruminococcaceae, and Marinilabiliaceae. Further, the most abundant methanogenic genera were Methanobrevibacter, Methanoculleus, and Methanosarcina. Our observations suggest that in samples processed with P1, the volatile fatty acids were not completely converted to methane. This is supported by the finding that enrichment cultures obtained with P2 included acetoclastic methanogens, which might have prevented the accumulation of acetic acid. We conclude that differences in the inoculum preparation may have a major influence on the outcome of enrichment cultures from the P. marginata larvae gut.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25961
Grosch Schroeder, B., Logroño, W., Nunes da Rocha, U., Harms, H., Nikolausz, M. (2022):
Enrichment of anaerobic microbial communities from midgut and hindgut of sun beetle larvae (Pachnoda marginata) on wheat straw: effect of inoculum preparation
Microorganisms 10 (4), art. 761