Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1073/pnas.2114799119
Licence creative commons licence
Title (Primary) Sulfur and methane oxidation by a single microorganism
Author Gwak, J.-H.; Awala, S.I.; Nguyen, N.-L.; Yu, W.-J.; Yang, H.-Y.; von Bergen, M.; Jehmlich, N. ORCID logo ; Kits, K.D.; Loy, A.; Dunfield, P.F.; Dahl, C.; Hyun, J.-H.; Rhee, S.-K.
Journal Proceedings of the National Academy of Sciences of the United States of America
Year 2022
Department MOLSYB
Volume 119
Issue 32
Page From e2114799119
Language englisch
Topic T9 Healthy Planet
Abstract Natural and anthropogenic wetlands are major sources of the atmospheric greenhouse gas methane. Methane emissions from wetlands are mitigated by methanotrophic bacteria at the oxic–anoxic interface, a zone of intense redox cycling of carbon, sulfur, and nitrogen compounds. Here, we report on the isolation of an aerobic methanotrophic bacterium, ‘Methylovirgula thiovorans' strain HY1, which possesses metabolic capabilities never before found in any methanotroph. Most notably, strain HY1 is the first bacterium shown to aerobically oxidize both methane and reduced sulfur compounds for growth. Genomic and proteomic analyses showed that soluble methane monooxygenase and XoxF-type alcohol dehydrogenases are responsible for methane and methanol oxidation, respectively. Various pathways for respiratory sulfur oxidation were present, including the Sox–rDsr pathway and the S4I system. Strain HY1 employed the Calvin–Benson–Bassham cycle for CO2 fixation during chemolithoautotrophic growth on reduced sulfur compounds. Proteomic and microrespirometry analyses showed that the metabolic pathways for methane and thiosulfate oxidation were induced in the presence of the respective substrates. Methane and thiosulfate could therefore be independently or simultaneously oxidized. The discovery of this versatile bacterium demonstrates that methanotrophy and thiotrophy are compatible in a single microorganism and underpins the intimate interactions of methane and sulfur cycles in oxic–anoxic interface environments.
Persistent UFZ Identifier
Gwak, J.-H., Awala, S.I., Nguyen, N.-L., Yu, W.-J., Yang, H.-Y., von Bergen, M., Jehmlich, N., Kits, K.D., Loy, A., Dunfield, P.F., Dahl, C., Hyun, J.-H., Rhee, S.-K. (2022):
Sulfur and methane oxidation by a single microorganism
Proc. Natl. Acad. Sci. U.S.A. 119 (32), e2114799119