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Title (Primary) Exploring the extent of phosphorus and heavy metal uptake by single cells of Saccharomyces cerevisiae and their effects on intrinsic elements by SC-ICP-TOF-MS
Author Qin, W.; Stärk, H.-J.; Müller, S.; Reemtsma, T.
Journal Frontiers in Microbiology
Year 2022
Department UMB; ANA
Volume 13
Page From art. 870931
Language englisch
Topic T9 Healthy Planet
Supplements https://ndownloader.figstatic.com/files/34890654
Keywords Elemental distribution; Transition metals, Phosphorus; Absorption; Toxicity; Adsorption
Abstract The effect of six heavy metals, Ag, Pb, Pd, Cu, Ni and Cr, on P uptake by yeast was investigated by single-cell analysis using inductively coupled plasma time-of-flight mass spectrometry (SC-ICP-TOF-MS). It was found that the P content in cells with 1.55 g L-1 P feeding after P starvation was increased by ~ 70 % compared to control cells. Heavy metals at 10 ppm, except Cu, had a negative impact on P accumulation by cells. Pd reduced the P content by 26 % in single cells compared to control cells. Metal uptake was strongest for Ag and Pd (0.7 × 10-12 L cell-1) and weakest for Cr (0.05 × 10-12 L cell-1). Exposure to Cr markedly reduced (- 50 %) Mg in cells and had the greatest impact on the intrinsic element composition. The SC-ICP-TOF-MS shows the diversity of elemental content in single cells: for example, the P content under standard conditions varied between 12.4 to 890 fg cell-1. This technique allows to study both, the uptake of elements and sublethal effects on the physiology at a single cell level.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25929
Qin, W., Stärk, H.-J., Müller, S., Reemtsma, T. (2022):
Exploring the extent of phosphorus and heavy metal uptake by single cells of Saccharomyces cerevisiae and their effects on intrinsic elements by SC-ICP-TOF-MS
Front. Microbiol. 13 , art. 870931