Publication Details

Category Text Publication
Reference Category Conference papers
DOI 10.5194/egusphere-egu2020-5784
Licence creative commons licence
Title (Primary) Towards soil micro-zymography: comparison of staining and impregnation strategies
Title (Secondary) EGU General Assembly, EGU2020, Online, 4-8 May 2020
Author Ghaderi, N.; Guber, A.; Schmidt, H.; Blagodatskaya, E.
Source Titel EGUsphere
Year 2020
Department BOOEK
Page From EGU2020-5784
Language englisch
Keywords enzyme activity; zymography; fluorogenic substrates
Abstract Enzymes are produced by microorganisms either intracellularly in cell’s cytoplasm and periplasm or extracellularly either as attached to outer surface of cell membranes or released to the soil microhabitats. The distribution of microhabitats in soil is highly heterogeneous with high abundance of microorganisms in the small volume of soil hotspots, e.g., in the rhizosphere - the most important plant-soil interface with very dynamic interactions between roots and microorganisms. Soil zymography is one of the most realistic methods developed to visualize enzyme activity in undisturbed soil at the mesoscale (mm-cm) level using substrate-saturated membranes. However, visualization of enzymatic processes at the micro-scale level remains a challenge. We tested several impregnation strategies of soil sample (e.g., by agarose gel, silicon spray and super transparent silicon mixture) for their suitability for micro-zymography, i.e., for visualization of enzyme activity in undisturbed soil particles at the microscopic level combining fluorogenic substrates with epifluorescence microscopy. The pros- and cons- of various combinations of impregnation and staining of micro-sized soil samples will be discussed.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25799
Ghaderi, N., Guber, A., Schmidt, H., Blagodatskaya, E. (2020):
Towards soil micro-zymography: comparison of staining and impregnation strategies
EGU General Assembly, EGU2020, Online, 4-8 May 2020
EGUsphere
Copernicus Publications, EGU2020-5784 10.5194/egusphere-egu2020-5784