Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1038/s41598-022-06558-0 |
Licence  |
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| Title (Primary) | Extracellular degradation of a polyurethane oligomer involving outer membrane vesicles and further insights on the degradation of 2,4-diaminotoluene in Pseudomonas capeferrum TDA1 |
| Author | Puiggené, Ò.; Cárdenas Espinosa, M.J.; Schlosser, D.
; Thies, S.; Jehmlich, N.
; Kappelmeyer, U.
; Schreiber, S.
; Wibberg, D.; Kalinowski, J.; Harms, H.; Heipieper, H.J.
; Eberlein, C.
|
| Source Titel | Scientific Reports |
| Year | 2022 |
| Department | UMB; UBT; MOLSYB; BIOINF |
| Volume | 12 |
| Page From | art. 2666 |
| Language | englisch |
| Topic | T7 Bioeconomy T9 Healthy Planet |
| Supplements | Supplement 1 |
| UFZ wide themes | Microplastics; |
| Abstract | The continuing reports of plastic pollution in various ecosystems highlight the threat posed by the ever-increasing consumption of synthetic polymers. Therefore, Pseudomonas capeferrum TDA1, a strain recently isolated from a plastic dump site, was examined further regarding its ability to degrade polyurethane (PU) compounds. The previously reported degradation pathway for 2,4-toluene diamine, a precursor and degradation intermediate of PU, could be confirmed by RNA-seq in this organism. In addition, different cell fractions of cells grown on a PU oligomer were tested for extracellular hydrolytic activity using a standard assay. Strikingly, purified outer membrane vesicles (OMV) of P. capeferrum TDA1 grown on a PU oligomer showed higher esterase activity than cell pellets. Hydrolases in the OMV fraction possibly involved in extracellular PU degradation were identified by mass spectrometry. On this basis, we propose a model for extracellular degradation of polyester-based PUs by P. capeferrum TDA1 involving the role of OMVs in synthetic polymer degradation. |
| Puiggené, Ò., Cárdenas Espinosa, M.J., Schlosser, D., Thies, S., Jehmlich, N., Kappelmeyer, U., Schreiber, S., Wibberg, D., Kalinowski, J., Harms, H., Heipieper, H.J., Eberlein, C. (2022): Extracellular degradation of a polyurethane oligomer involving outer membrane vesicles and further insights on the degradation of 2,4-diaminotoluene in Pseudomonas capeferrum TDA1 Sci. Rep. 12 , art. 2666 10.1038/s41598-022-06558-0 |
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