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Title (Primary) Extracellular degradation of a polyurethane oligomer involving outer membrane vesicles and further insights on the degradation of 2,4-diaminotoluene in Pseudomonas capeferrum TDA1
Author Puiggené, Ò.; Cárdenas Espinosa, M.J.; Schlosser, D.; Thies, S.; Jehmlich, N.; Kappelmeyer, U.; Schreiber, S.; Wibberg, D.; Kalinowski, J.; Harms, H.; Heipieper, H.J.; Eberlein, C.
Journal Scientific Reports
Year 2022
Department UMB; UBT; MOLSYB
Volume 12
Page From art. 2666
Language englisch
Topic T7 Bioeconomy
T9 Healthy Planet
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-022-06558-0/MediaObjects/41598_2022_6558_MOESM1_ESM.docx
Abstract The continuing reports of plastic pollution in various ecosystems highlight the threat posed by the ever-increasing consumption of synthetic polymers. Therefore, Pseudomonas capeferrum TDA1, a strain recently isolated from a plastic dump site, was examined further regarding its ability to degrade polyurethane (PU) compounds. The previously reported degradation pathway for 2,4-toluene diamine, a precursor and degradation intermediate of PU, could be confirmed by RNA-seq in this organism. In addition, different cell fractions of cells grown on a PU oligomer were tested for extracellular hydrolytic activity using a standard assay. Strikingly, purified outer membrane vesicles (OMV) of P. capeferrum TDA1 grown on a PU oligomer showed higher esterase activity than cell pellets. Hydrolases in the OMV fraction possibly involved in extracellular PU degradation were identified by mass spectrometry. On this basis, we propose a model for extracellular degradation of polyester-based PUs by P. capeferrum TDA1 involving the role of OMVs in synthetic polymer degradation.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25713
Puiggené, Ò., Cárdenas Espinosa, M.J., Schlosser, D., Thies, S., Jehmlich, N., Kappelmeyer, U., Schreiber, S., Wibberg, D., Kalinowski, J., Harms, H., Heipieper, H.J., Eberlein, C. (2022):
Extracellular degradation of a polyurethane oligomer involving outer membrane vesicles and further insights on the degradation of 2,4-diaminotoluene in Pseudomonas capeferrum TDA1
Sci. Rep. 12 , art. 2666