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Title (Primary) Physiological effects of 2-bromoethanesulfonate on hydrogenotrophic pure and mixed cultures
Author Logroño, W.; Nikolausz, M.; Harms, H.; Kleinsteuber, S.
Journal Microorganisms
Year 2022
Department UMB
Volume 10
Issue 2
Page From art. 355
Language englisch
Topic T7 Bioeconomy
Supplements https://www.mdpi.com/2076-2607/10/2/355/s1
Keywords formic acid; methanogenesis; homoacetogenesis; acetic acid; formate dehydrogenase; formate synthase; power to gas; biological biogas upgrading; biomethane
Abstract Mixed or pure cultures can be used for biomethanation of hydrogen. Sodium 2- 9 bromoethanesulfonate (BES) is an inhibitor of methanogenesis used to investigate competing reactions like homoacetogenesis in mixed cultures. To understand the effect of BES on the hydrogenotrophic metabolism in a biomethanation process, anaerobic granules from a wastewater treatment plant, a hydrogenotrophic enrichment culture, and pure cultures of Methanococcus maripaludis and Methanobacterium formicicum were incubated under H2/CO2 headspace in the presence or absence of BES, and the turnover of H2, CO2, CH4, formate, and acetate was analyzed. Anaerobic granules produced the highest amount of formate after 24 h of incubation in the presence of BES. Treating the enrichment culture with BES led to accumulation of formate. M. maripaludis
produced more formate than M. formicicum when treated with BES. The non-inhibited methanogenic communities produced small amounts of formate whereas the pure cultures did not. The highest amount of acetate was produced by the anaerobic granules concomitantly with formate consumption. These results indicate that formate is an important intermediate of hydrogenotrophic metabolism accumulating upon methanogenesis inhibition.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25709
Logroño, W., Nikolausz, M., Harms, H., Kleinsteuber, S. (2022):
Physiological effects of 2-bromoethanesulfonate on hydrogenotrophic pure and mixed cultures
Microorganisms 10 (2), art. 355