Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1007/s00253-023-12375-w
Licence creative commons licence
Title (Primary) Effects of reducing, stabilizing, and antibiotic agents on “Candidatus Kuenenia stuttgartiensis”
Author Ude, E.O.; Haas, J.; Kaiyoum, M.K.; Ding, C.; Adrian, L.
Source Titel Applied Microbiology and Biotechnology
Year 2023
Department UBT
Volume 107
Issue 5-6
Page From 1829
Page To 1843
Language englisch
Topic T7 Bioeconomy
Keywords Anammox bacteria; Antibiotics; Online monitoring; Planktonic cell; Reducing agents; Sulfite
Abstract Anaerobic ammon ium oxidizing (anammox) bacteria oxidize ammonium and reduce nitrite, producing N2, and could play a major role in energy-optimized wastewater treatment. However, sensitivity to various environmental conditions and slow growth currently hinder their wide application. Here, we attempted to determine online the effect of environmental stresses on anammox bacteria by using an overnight batch activity test with whole cells, in which anammox activity was calculated by quantifying N2 production via headspace-pressure monitoring. A planktonic mixed culture dominated by “Candidatus Kuenenia stuttgartiensis” strain CSTR1 was cultivated in a 30-L semi-continuous stirring tank reactor. In overnight resting-cell anammox activity tests, oxygen caused strong inhibition of anammox activity, which was reversed by sodium sulfite (30 µM). The tested antibiotics sulfamethoxazole, kanamycin, and ciprofloxacin elicited their effect on a dose-dependent manner; however, strain CSTR1 was highly resistant to sulfamethoxazole. Anammox activity was improved by activated carbon and Fe2O3. Protein expression analysis from resting cells after anammox activity stimulation revealed that NapC/NirT family cytochrome c (KsCSTR_12840), hydrazine synthase, hydrazine dehydrogenase, hydroxylamine oxidase, and nitrate:nitrite oxidoreductase were upregulated, while a putative hydroxylamine oxidoreductase HAO (KsCSTR_49490) was downregulated. These findings contribute to the growing knowledge on anammox bacteria physiology, eventually leading to the control of anammox bacteria growth and activity in real-world application.
Persistent UFZ Identifier
Ude, E.O., Haas, J., Kaiyoum, M.K., Ding, C., Adrian, L. (2023):
Effects of reducing, stabilizing, and antibiotic agents on “Candidatus Kuenenia stuttgartiensis”
Appl. Microbiol. Biotechnol. 107 (5-6), 1829 - 1843